CHIRAL SEPARATION BASED ON IMMOBILIZED INTACT AND FRAGMENTED CELLOBIOHYDROLASE-II (CBH-II) - A COMPARISON WITH CELLOBIOHYDROLASE-I (CBH-I)

Intact and fragmented cellobiohydrolase II (CBH II) were immobilized to silica and used as chiral stationary phases (CSPs) for liquid chromatographic separations of enantiomers. Both acidic and basic chiral compounds could be resolved into their enantiomers on these phases, The enantioselectivities obtained on intact CBH II and its core were almost equivalent. Comparisons were also made with CBH I silica. It was found that the new materials show quite different chiral and chromatographic properties, The enzymatic activity of the CBH II in free solution was influenced by alprenolol and mexiletine, both separated on the corresponding CSP. It indicates that the sites for catalysis and for chiral recognition overlap. (C) 1995 Wiley-Liss, Inc.