DISTRIBUTION OF SUBSTANCE-P-IMMUNOREACTIVE ELEMENTS IN THE PREOPTIC AREA AND THE HYPOTHALAMUS OF THE RAT

被引:69
作者
LARSEN, PJ
机构
[1] Institute of Medical Anatomy, Department B, University of Copenhagen, Copenhagen
关键词
IMMUNOHISTOCHEMISTRY; TACHYKININS; CIRCUMVENTRICULAR ORGANS; PARAVENTRICULAR NUCLEUS; NEURONAL CONNECTIVITY; SEXUAL BEHAVIOR; WATER HOMEOSTASIS;
D O I
10.1002/cne.903160304
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The localization and morphology of neurons, processes, and neuronal groups in the rat preoptic area and hypothalamus containing substance P-like immunoreactivity were studied with a highly selective antiserum raised against synthetic substance P. The antiserum was thoroughly characterized by immunoblotting; only substance P was recognized by the antiserum. Absorption of the antiserum with synthetic substance P abolished immunostaining while addition of other hypothalamic neuropeptides had no effect on the immunostaining. The specificity of the observed immunohistochemical staining pattern was further confirmed with a monoclonal substance P antiserum. The distribution of substance P immunoreactive perikarya was investigated in colchicine-treated animals, whereas the distribution of immunoreactive nerve fibers and terminals was described in brains from untreated animals. In colchicine-treated rats, immunoreactive cells were reliably detected throughout the preoptic area and the hypothalamus. In the preoptic region, labeled cells were found in the anteroventral periventricular and the anteroventral preoptic nuclei and the medial and lateral preoptic areas. Within the hypothalamus, immunoreactive cells were found in the suprachiasmatic, paraventricular, supraoptic, ventromedial, dorsomedial, supramammillary, and premammillary nuclei, the retrochiasmatic, medial hypothalamic, and lateral hypothalamic areas, and the tuber cinereum. The immunoreactive cell groups were usually continuous with adjacent cell groups. Because of the highly variable effect of the colchicine treatment, it was not possible to determine the actual number of immunoreactive cells. Mean soma size varied considerably from one cell group to another. Cells in the magnocellular subnuclei of the paraventricular and supraoptic nuclei were among the largest, with a diameter of about 25-mu-m, while cells in the supramammillary and suprachiasmatic nuclei were among the smallest, with a diameter of about 12-mu-m. Immunoreactive nerve fibers were found in all areas of the preoptic area and the hypothalamus. The morphology, size, density, and number of terminals varied considerably from region to region. Thus, some areas contained single immunoreactive fibers, while others were innervated with such a density that individual nerve fibers were hardly discernible. During the last decade, knowledge about neural organization of rodent hypothalamic areas and mammalian tachykinin biochemistry has increased substantially. In the light of these new insights, the present study gives comprehensive morphological evidence that substance P may be centrally involved in a wide variety of hypothalamic functions. Among these could be sexual behavior, pituitary hormone release, and water homeostasis.
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页码:287 / 313
页数:27
相关论文
共 120 条
[51]  
LARSEN PJ, 1989, J CHEM NEUROANAT, V2, P83
[52]   EFFERENT PROJECTIONS FROM THE PERIVENTRICULAR AND MEDIAL PARVICELLULAR SUBNUCLEI OF THE HYPOTHALAMIC PARAVENTRICULAR NUCLEUS TO CIRCUMVENTRICULAR ORGANS OF THE RAT - A PHASEOLUS-VULGARIS-LEUKOAGGLUTININ (PHA-L) TRACING STUDY [J].
LARSEN, PJ ;
MOLLER, M ;
MIKKELSEN, JD .
JOURNAL OF COMPARATIVE NEUROLOGY, 1991, 306 (03) :462-479
[53]   BINDING OF A IODINATED SUBSTANCE-P ANALOG TO A NK-1 RECEPTOR ON ISOLATED CELL-MEMBRANES FROM RAT ANTERIOR-PITUITARY [J].
LARSEN, PJ ;
MIKKELSEN, JD ;
SAERMARK, T .
ENDOCRINOLOGY, 1989, 124 (05) :2548-2557
[54]   BINDING AND INTERNALIZATION OF A IODINATED SUBSTANCE-P ANALOG BY CULTURED ANTERIOR-PITUITARY CELLS [J].
LARSEN, PJ ;
MIKKELSEN, JD ;
MAU, S ;
SAERMARK, T .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1989, 65 (1-2) :91-101
[55]  
LARSEN PJ, 1989, 4TH P INT C NEUR, P4
[56]   EVIDENCE FOR OPIATE RECEPTORS ON PITUICYTES [J].
LIGHTMAN, SL ;
NINKOVIC, M ;
HUNT, SP ;
IVERSEN, LL .
NATURE, 1983, 305 (5931) :235-237
[57]   ATRIAL-NATRIURETIC-FACTOR IS RELEASED INTO HYPOPHYSEAL PORTAL BLOOD - DIRECT EVIDENCE THAT ATRIAL-NATRIURETIC-FACTOR MAY BE A NEUROHORMONE INVOLVED IN HYPOTHALAMIC PITUITARY CONTROL [J].
LIM, AT ;
SHEWARD, WJ ;
COPOLOV, D ;
WINDMILL, D ;
FINK, G .
JOURNAL OF NEUROENDOCRINOLOGY, 1990, 2 (01) :15-18
[58]   DISTRIBUTION OF SUBSTANCE P-LIKE IMMUNOREACTIVITY IN CENTRAL NERVOUS-SYSTEM OF RAT .1. CELL BODIES AND NERVE-TERMINALS [J].
LJUNGDAHL, A ;
HOKFELT, T ;
NILSSON, G .
NEUROSCIENCE, 1978, 3 (10) :861-+
[59]   THE CORTICO-MEDIAL AMYGDALA IN THE CENTRAL NERVOUS-SYSTEM ORGANIZATION OF AGONISTIC BEHAVIOR [J].
LUITEN, PGM ;
KOOLHAAS, JM ;
DEBOER, S ;
KOOPMANS, SJ .
BRAIN RESEARCH, 1985, 332 (02) :283-297
[60]   TACHYKININS [J].
MAGGIO, JE .
ANNUAL REVIEW OF NEUROSCIENCE, 1988, 11 :13-28