IN-VIVO N-15 NMR-STUDIES OF SECONDARY METABOLISM IN TRANSFORMED ROOT CULTURES OF DATURA-STRAMONIUM AND NICOTIANA-TABACUM

被引:24
作者
FORD, YY
FOX, GG
RATCLIFFE, RG
ROBINS, RJ
机构
[1] UNIV OXFORD, DEPT PLANT SCI, OXFORD OX1 3RB, ENGLAND
[2] INST FOOD RES, NORWICH LAB, NORWICH NR4 7UA, NORFOLK, ENGLAND
关键词
DATURA STRAMONIUM; NICOTIANA TABACUM; SOLANACEAE; TRANSFORMED ROOTS; IN VIVO N-15 NMR; SECONDARY METABOLISM;
D O I
10.1016/S0031-9422(00)97071-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In vivo N-15 NMR spectra of N-15-labelled Datura stramonium and Nicotiana tabacum transformed root cultures have been obtained. It is shown for the first time that nitrogenous secondary metabolites can be detected using in vivo N-15 NMR, and that information can be deduced on the subcellular distribution of nicotine.
引用
收藏
页码:333 / 339
页数:7
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