RNA-SYNTHESIS IN ISOLATED-MITOCHONDRIA FROM BRAIN CORTEX, CEREBELLUM AND STEM - EVIDENCE OF DIFFERENT TRANSCRIPTIONAL RATES

被引:7
作者
ENRIQUEZ, JA [1 ]
PEREZMARTOS, A [1 ]
FERNANDEZSILVA, P [1 ]
LOPEZPEREZ, MJ [1 ]
MONTOYA, J [1 ]
机构
[1] UNIV ZARAGOZA,FAC VET,DEPT BIOQUIM & BIOL MOLEC & CELULAR,E-50013 ZARAGOZA,SPAIN
来源
INTERNATIONAL JOURNAL OF BIOCHEMISTRY | 1993年 / 25卷 / 12期
关键词
D O I
10.1016/0020-711X(88)90330-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
1. A system for studying RNA synthesis in isolated sheep brain mitochondria was set up to investigate the transcriptional activity of different brain regions (cortex, cerebellum and brain stem). in this system, mitochondrial DNA is transcribed and RNA processed in a way that faithfully reproduces the in vivo process. 2. The comparison of the electrophoretic patterns of the mitochondrial DNA transcription products showed that although they were qualitatively similar, there were large differences in the rate of mitochondrial DNA transcription of the three regions studied, cerebellum and brain stem showing transcriptional rates which were 34 and 18% respectively of that of cerebral cortex.
引用
收藏
页码:1951 / 1956
页数:6
相关论文
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