HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR POTENCY DETERMINATION OF CEPHALEXIN IN COMMERCIAL PREPARATIONS AND FOR STABILITY STUDIES

被引：20

作者：

HSU, MC ^{[1
]}

LIN, YS ^{[1
]}

CHUNG, HC ^{[1
]}

机构：

[1] DEPT HLTH,NATL LABS FOODS & DRUGS,TAIPEI 11513,TAIWAN

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 692卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(94)00688-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reversed-phase column liquid chromatographic method was developed for the assay of cephalexin in bulk drugs and pharmaceutical preparations. An equation was derived showing a linear relationship between peak-area ratio of cephalexin to acetaminophen (internal standard) and the cephalexin concentration over a range of 0.2-1.75 mg/ml (r=0.9999). Standard addition recoveries were generally greater than 99.5%. The relative standard deviations were between 0.38 and 0.63% in the within-day assay, and 1.05% in the between-day assay. The column liquid chromatographic assay results were compared with those obtained from a microbiological assay, which indicated that the proposed method is a suitable substitute for the microbiological method for potency assays and stability studies of cephalexin preparations.

引用

页码：67 / 72

页数：6