ENHANCEMENT OF MONOCLONAL-ANTIBODY YIELD BY HYBRIDOMA FED-BATCH CULTURE, RESULTING IN EXTENDED MAINTENANCE OF VIABLE CELL-POPULATION

被引：20

作者：

BUSHELL, ME

BELL, SL

SCOTT, MF

SPIER, RE

WARDELL, JN

SANDERS, PG

机构：

[1] School of Biological Science, University of Surrey, Guildford, Surrey

[2] Kabi Pharmacia, Stockholm, S-11287

[3] Wellcome Research Laboratories, Beckenham, Kent, BR3 3BS, Langley Court

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1994年 / 44卷 / 09期

关键词：

HYBRIDOMA; FED BATCH; MATERIALS BALANCING; FERMENTATION;

D O I：

10.1002/bit.260440911

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Hybridoma batch cultures were extended using feed formulations based on nutrient consumption measured during different batch culture phases when (a) growth but negligible antibody production was taking place; (b) maximum antibody production rate and declining viable cell growth rate were observed. Strategy (a) was the more successful (2.8-fold compared with 1.8-fold antibody titer increase) and maintained cell viability for longer. Analysis of the effects of omitting individual amino acids yielded results which were consistent with those from the feeding experiment. (C) 1994 John Wiley & Sons, Inc.

引用

页码：1099 / 1106

页数：8

共 24 条

[1] A STRUCTURED MODEL FOR MONOCLONAL-ANTIBODY SYNTHESIS IN EXPONENTIALLY GROWING AND STATIONARY PHASE HYBRIDOMA CELLS [J].