FREQUENCY ANALYSES OF ACTIVE NORS IN NUCLEI OF ARTIFICIALLY INDUCED TRIPLOID FISHES

被引：16

作者：

FLAJSHANS, M

RAB, P

DOBOSZ, S

机构：

[1] CZECHOSLOVAK ACAD SCI, INST ANIM PHYSIOL & GENET, DEPT GENET, CS-27721 LIBECHOV, CZECHOSLOVAKIA

[2] INLAND FISHERIES INST, SALMONID RES LAB, PL-83330 ZUKOWO, POLAND

来源：

THEORETICAL AND APPLIED GENETICS | 1992年 / 85卷 / 01期

关键词：

ACTIVE NORS; ARTIFICIAL TRIPLOIDY; RAINBOW TROUT; COMMON CARP; TENCH; MINIMUM NECESSARY SAMPLE SIZE;

D O I：

10.1007/BF00223846

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The correspondence between increased numbers of both chromosomal and nuclear NORs and artificially induced triploidy in three fish species (rainbow trout, Oncorhynchus mykiss; common carp, Cyprinus carpio; and tench, Tinca tinca) has been confirmed by CMA3 fluorescence and Ag-staining. The frequencies of cell nuclei with one, two and three active NORs, as revealed by Ag-staining, has been analyzed statistically to find the minimum cell number which verifies the increased ploidy level. A minimum sample size of about 80 cells exhibiting three active NORs is sufficient to confirm triploidy in all three species and may be of use for categorising other ploidy-manipulated fish species.

引用

页码：68 / 72

页数：5

共 24 条

[1] CYTOGENETIC STUDIES IN NORTH-AMERICAN MINNOWS (CYPRINIDAE) .17. CHROMOSOMAL NOR PHENOTYPES OF 12 SPECIES, WITH COMMENTS ON CYTOSYSTEMATIC RELATIONSHIPS AMONG 50 SPECIES [J].