A YEAST-ESCHERICHIA-COLI SHUTTLE VECTOR CONTAINING THE M13 ORIGIN OF REPLICATION

被引：14

作者：

RHODES, N

COMPANY, M

ERREDE, B

机构：

[1] Department of Chemistry, University of North Carolina, Chapel Hill

来源：

PLASMID | 1990年 / 23卷 / 02期

关键词：

D O I：

10.1016/0147-619X(90)90036-C

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A yeast-Escherichia coli shuttle vector containing the M13 origin of replication has been constructed. This vector allows selection and replication in both Saccharyomyces cerevisiae and E. coli, as well as single-stranded packaging from E. coli upon infection with a helper phage. The presence of a polylinker with various unique restriction sites facilitates the cloning of desired genes. © 1990.

引用

页码：159 / 162

页数：4

共 9 条

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