A NEW BIOSENSOR FOR SPECIFIC DETERMINATION OF GLUCOSE OR FRUCTOSE USING AN OXIDOREDUCTASE OF ZYMOMONAS-MOBILIS

被引：16

作者：

PARK, JK ^{[1
]}

KIM, HS ^{[1
]}

机构：

[1] KOREA INST SCI & TECHNOL, DEPT BIOL SCI & ENGN, POB 150, SEOUL 130650, SOUTH KOREA

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1990年 / 36卷 / 07期

关键词：

D O I：

10.1002/bit.260360713

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cells of Zymomonas mobilis were permeabilized with toluene in order to utilize the enzymes, glucose–fructose oxidoreductase and gluconolactonase, inside the intact cells. Permeabilized cells were immobilized in a gelatin membrane, and a whole cell enzyme electrode was constructed by fixing the membrane on pH electrode. The biosensor developed was used for specific determination of glucose or fructose by detecting the production rate of hydrogen ion. Optimum conditions for biosensor response were pH 6.2 and temperature of 39 °C. The biosensor was highly specific and reproducible, and calibration curves for glucose and fructose were excellent, being linear up to 5 and 50 g/L, respectively. Copyright © 1990 John Wiley & Sons, Inc.

引用

页码：744 / 749

页数：6

共 15 条

[1] THE SIMULTANEOUS PRODUCTION OF SORBITOL FROM FRUCTOSE AND GLUCONIC ACID FROM GLUCOSE USING AN OXIDOREDUCTASE OF ZYMOMONAS-MOBILIS [J].

CHUN, UH ;

ROGERS, PL .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1988, 29 (01) :19-24

[2] EXTERNALLY BUFFERED ENZYME ELECTRODE FOR DETERMINATION OF GLUCOSE [J].

CLELAND, N ;

ENFORS, SO .

ANALYTICAL CHEMISTRY, 1984, 56 (11) :1880-1884

[3] OXYGEN-STABILIZED ENZYME ELECTRODE FOR D-GLUCOSE ANALYSIS IN FERMENTATION BROTHS [J].

ENFORS, SO .

ENZYME AND MICROBIAL TECHNOLOGY, 1981, 3 (01) :29-32

[4] ENZYME ELECTRODE FOR AMPEROMETRIC DETERMINATION OF GLUCOSE [J].

GUILBAULT, GG ;

LUBRANO, GJ .

ANALYTICA CHIMICA ACTA, 1973, 64 (03) :439-455

[5] GLUCOSE SENSOR USING IMMOBILIZED WHOLE CELLS OF PSEUDOMONAS-FLUORESCENS [J].

KARUBE, I ;

MITSUDA, S ;

SUZUKI, S .

EUROPEAN JOURNAL OF APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1979, 7 (04) :343-350

[6]

Kim W. J., UNPUB

[7] MICROBIAL ENZYMES IN BIOSENSORS [J].

MULLEN, WH ;

VADGAMA, PM .

JOURNAL OF APPLIED BACTERIOLOGY, 1986, 61 (03) :181-193

[8] DETERMINATION OF GLUCOSE, UREA AND PENICILLIN USING ENZYME-PH-ELECTRODES [J].

NILSSON, H ;

AKERLUND, AC ;

MOSBACH, K .

BIOCHIMICA ET BIOPHYSICA ACTA, 1973, 320 (02) :529-534

[9] ENZYME ELECTRODE FOR SPECIFIC DETERMINATION OF L-LYSINE [J].

ROMETTE, JL ;

YANG, JS ;

KUSAKABE, H .

BIOTECHNOLOGY AND BIOENGINEERING, 1983, 25 (11) :2557-2566

[10] GLUCOSE-OXIDASE ELECTRODE - MEASUREMENTS OF GLUCOSE IN SAMPLES EXHIBITING HIGH VARIABILITY IN OXYGEN-CONTENT [J].

ROMETTE, JL ;

FROMENT, B ;

THOMAS, D .

CLINICA CHIMICA ACTA, 1979, 95 (02) :249-253

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