SYNAPTIC PHARMACOLOGY OF THE SUPERIOR OLIVARY COMPLEX STUDIED IN MOUSE-BRAIN SLICE

被引:74
作者
WU, SH [1 ]
KELLY, JB [1 ]
机构
[1] CARLETON UNIV,DEPT PSYCHOL,SENSORY NEUROSCI LAB,OTTAWA K1S 5B6,ONTARIO,CANADA
关键词
D O I
10.1523/JNEUROSCI.12-08-03084.1992
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The synaptic pharmacology of the lateral superior olive (LSO) and medial nucleus of the trapezoid body (MNTB) was examined in a brain slice preparation of the mouse superior olivary complex (SOC). Physiological responses in SOC were elicited by electrical stimulation of the trapezoid body ipsilateral or contralateral to the recording site, and bilateral interactions were investigated by combined ipsilateral and contralateral stimulation. Pharmacological effects were tested by bath application of amino acid agonists and antagonists. Neurons in MNTB were excited by contralateral stimulation and unaffected by ipsilateral stimulation. Excitatory amino acid (EAA) agonists-kainic acid (KA), quisqualic acid (QA), or L-glutamate-caused spontaneous firing at low concentrations and eliminated responses at higher concentrations in MNTB. The EAA agonist NMDA had relatively little effect at comparable concentrations. Stimulus-elicited responses were blocked by non-NMDA antagonists 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and 6,7-dinitroquinoxaline-2,3-dione (DNQX) and by the nonspecific EAA antagonist kynurenic acid, but were unaffected by the NMDA antagonist D,L-2-amino-5-phosphonovaleric acid (APV). LSO neurons were typically excited by ipsilateral stimulation and inhibited by contralateral stimulation. In LSO, KA, QA, and L-glutamate caused spontaneous firing at low concentrations and eliminated responses at higher concentrations, and NMDA had relatively little effect. Excitatory responses in the vast majority of LSO neurons were blocked by CNQX, DNQX, or kynurenic acid. Some responses were also blocked by APV. LSO neurons were affected by glycine, and contralateral inhibition in LSO was completely blocked by strychnine. NMDA also blocked inhibition in LSO. These results indicate that excitation of both MNTB and LSO neurons is mediated primarily by an EAA neurotransmitter through non-NMDA receptors and that contralateral inhibition of LSO cells is mediated through strychnine-dependent glycine receptors. NMDA receptors may play a role in binaural processing by modulating contralateral inhibitory input to LSO.
引用
收藏
页码:3084 / 3097
页数:14
相关论文
共 96 条
[41]   MORPHOLOGICAL EVIDENCE FOR THE EXISTENCE OF MULTIPLE NEURONAL CLASSES IN THE CAT LATERAL SUPERIOR OLIVARY NUCLEUS [J].
HELFERT, RH ;
SCHWARTZ, IR .
JOURNAL OF COMPARATIVE NEUROLOGY, 1986, 244 (04) :533-549
[42]   QUINOXALINEDIONES - POTENT COMPETITIVE NON-NMDA GLUTAMATE RECEPTOR ANTAGONISTS [J].
HONORE, T ;
DAVIES, SN ;
DREJER, J ;
FLETCHER, EJ ;
JACOBSEN, P ;
LODGE, D ;
NIELSEN, FE .
SCIENCE, 1988, 241 (4866) :701-703
[43]   IONIC MECHANISMS ASSOCIATED WITH DEPOLARIZATION BY GLUTAMATE AND ASPARTATE ON HUMAN AND RAT SPINAL NEURONS IN TISSUE-CULTURE [J].
HOSLI, L ;
ANDRES, PF ;
HOSLI, E .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1976, 363 (01) :43-48
[44]  
IRVINE DRF, 1986, PROGR SENSORY PHYSL, V7
[45]   TRANSNEURONAL CHANGES OF SYNAPTIC ENDINGS AND NUCLEAR CHROMATIN IN TRAPEZOID BODY FOLLOWING COCHLEAR ABLATIONS IN CATS [J].
JEANBAPTISTE, M ;
MOREST, DK .
JOURNAL OF COMPARATIVE NEUROLOGY, 1975, 162 (01) :111-133
[46]   SOUND LOCALIZATION - EFFECTS OF UNILATERAL LESIONS IN CENTRAL AUDITORY-SYSTEM [J].
JENKINS, WM ;
MASTERTON, RB .
JOURNAL OF NEUROPHYSIOLOGY, 1982, 47 (06) :987-1016
[47]  
JESSELL TM, 1986, J EXP BIOL, V124, P239
[48]   GLYCINE POTENTIATES THE NMDA RESPONSE IN CULTURED MOUSE-BRAIN NEURONS [J].
JOHNSON, JW ;
ASCHER, P .
NATURE, 1987, 325 (6104) :529-531
[49]  
KAVANAGH GL, 1986, SOC NEUR ABSTR, V12, P1246
[50]   ANTAGONISTS OF GLUTAMINERGIC NEUROTRANSMISSION BLOCK RETINOTECTAL TRANSMISSION IN GOLDFISH [J].
LANGDON, RB ;
FREEMAN, JA .
BRAIN RESEARCH, 1986, 398 (01) :169-174