ESTABLISHMENT OF PIGMENTED CHOROIDAL MELANOMAS IN A RABBIT MODEL

被引：33

作者：

HU, LK ^{[1
]}

HUH, K ^{[1
]}

GRAGOUDAS, ES ^{[1
]}

YOUNG, LHY ^{[1
]}

机构：

[1] HARVARD UNIV, MASSACHUSETTS EYE & EAR INFIRM, SCH MED, 243 CHARLES ST, BOSTON, MA 02114 USA

来源：

RETINA-THE JOURNAL OF RETINAL AND VITREOUS DISEASES | 1994年 / 14卷 / 03期

关键词：

PIGMENTED CHOROIDAL MELANOMA; ANIMAL MODEL;

D O I：

10.1097/00006982-199414030-00014

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Purpose: To establish an animal model of pigmented choroidal melanoma. Methods: Four melanoma cell lines originally isolated from melanotic tumors (B16F10, RPMI 1846, OCM 1, and IIB) were used to establish choroidal melanomas in 105 rabbits; 88 animals were immunosuppressed with cyclosporine. Tumor cells were implanted transclerally and examined with indirect ophthalmoscopy, ultrasound, and photography. Results: Characteristic growth patterns were noted for each cell line. Animal cell lines typically produced choroidal tumors 3 to 4 mm in height within 2 weeks; human cell lines took an additional 7 to 10 days to achieve tumors of similar height. Tumors of heaviest pigmentation were generated consistently with the B16F10 cells, and with the other three cell lines only mild pigmentation was observed. Tumor shape varied depending on the source of implantation: diffuse, flat tumors were observed when cell suspensions were implanted, and nodular tumors were obtained with tumor fragments. Histopathologically, lesions were highly cellular, with rich vascularity and large numbers of mitotic figures. Conclusion: As the majority of human uveal melanomas are pigmented, the added feature of pigmentation associated with this model makes it more suitable for evaluating the role of newly developed phototherapies in the management of uveal melanoma.

引用

页码：264 / 269

页数：6

共 31 条

[11]

Liu L.H.S., Ni C., Hematoporphyrin phototherapy for experimental intraocular malignant melanoma, Arch Ophthalmol, 101, pp. 901-903, (1983)

[12]

Sery T.W., Dougherty T.J., Photoradiation of rabbit ocular malignant melanoma sensitized with hematoporphyrin derivative, Curr Eye Res, 3, pp. 519-528, (1984)

[13]

Burgess S.E.P., Chang S., Svitra P., Et al., Effect of hyperthermia on experimental choroidal melanoma, Br J Ophthalmol, 69, pp. 854-860, (1985)

[14]

Finger P.T., Packer S., Svitra P., Et al., Thermoradiotherapy for intraocular tumors, Arch Ophthalmol, 103, pp. 1574-1578, (1985)

[15]

Riedel K.G., Svitra P.P., Seddon J.M., Et al., Proton beam irradiation and hyperthermia: Effects on experimental choroidal melanoma, Arch Ophthalmol, 103, pp. 1862-1869, (1985)

[16]

Phillips A.M.R., Browne B.H., Allan D., Et al., Hematoporphyrin photosensitization treatment of experimental choroidal melanoma, Eye, 1, pp. 680-685, (1987)

[17]

Panagopoulos J.A., Svitra P.P., Puliafito C.A., Gragoudas E.S., Photodynamic therapy of experimental subchoroidal melanoma using chloroaluminum sulfonated phthalocyanine, Arch Ophthalmol, 107, pp. 886-890, (1987)

[18]

Jaffe G.J., Mieler W.F., Burke J.M., Williams G.A., Photoablation of ocular melanoma with a high-powered argon endolaser, Arch Ophthalmol, 107, pp. 113-118, (1989)

[19]

Mieler W.F., Jaffe G.J., Steeves R.A., Ferromagnetic hyperthermia and iodine 125 brachytherapy in the treatment of choroidal melanoma in a rabbit model, Arch Ophthalmol, 107, pp. 1524-1528, (1989)

[20]

Swift P.S., Stauffer P.R., Fries P.D., Et al., Microwave hyperthermia for choroidal melanoma in rabbits, Invest Ophthalmol Vis Sci, 31, pp. 1754-1760, (1990)

← 1 2 3 4 →