COMPARISON OF THE REACTIVITIES OF BACULOVIRUS-EXPRESSED RECOMBINANT NORWALK VIRUS CAPSID ANTIGEN WITH THOSE OF THE NATIVE NORWALK VIRUS-ANTIGEN IN SEROLOGIC ASSAYS AND SOME EPIDEMIOLOGIC OBSERVATIONS

被引:172
作者
GREEN, KY [1 ]
LEW, JF [1 ]
XI, J [1 ]
KAPIKIAN, AZ [1 ]
ESTES, MK [1 ]
机构
[1] BAYLOR COLL MED, DIV MOLEC VIROL, HOUSTON, TX 77030 USA
关键词
D O I
10.1128/JCM.31.8.2185-2191.1993
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Since the discovery of the Norwalk virus (NV) by immune electron microscopy (IEM) in 1972, serologic studies with this virus have relied on particle-positive fecal material from infected volunteers as the source of antigen because it has not been possible to propagate this virus in cell culture. However, the recent cloning of the W (strain 8FIIa) genome and expression of the capsid protein in a baculovirus system to form ''virus-like particles'' has provided a consistent source of antigen (designated rNV). The purpose of the present study was to compare the antigenicities of these rW particles with those of native W antigen derived from human fecal material by using well-characterized sera obtained from earlier studies. In IEM studies, the rNV antigen reacted with NV-specific antibodies in a manner similar to that observed previously when particle-positive fecal material was used as antigen. In addition, a direct enzyme-linked immunosorbent assay, in which the rW antigen was used as antigen, proved efficient and specific for the detection of serologic responses to W compared with the previously established techniques of IEM and blocking antibody immunoassays in which particle-positive fecal material was used as the antigen. The availability of an unlimited source of antigen will enable serologic studies that will greatly increase our understanding of the epidemiology of W and its role in human enteric illness.
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页码:2185 / 2191
页数:7
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