Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles

被引：2230

作者：

Lotvall, Jan ^{[1
]}

Hill, Andrew F. ^{[2
]}

Hochberg, Fred ^{[3
]}

Buzas, Edit I. ^{[4
]}

Di Vizio, Dolores ^{[5
]}

Gardiner, Christopher ^{[6
]}

Gho, Yong Song ^{[7
]}

Kurochkin, Igor V. ^{[8
]}

Mathivanan, Suresh ^{[9
]}

Quesenberry, Peter ^{[10
]}

Sahoo, Susmita ^{[11
]}

Tahara, Hidetoshi ^{[12
]}

Wauben, Marca H. ^{[13
]}

Witwer, Kenneth W. ^{[14
]}

Thery, Clotilde ^{[15
]}

机构：

[1] Univ Gothenburg, Krefting Res Ctr, Gothenburg, Sweden

[2] Univ Melbourne, Dept Biochem & Mol Biol, Mol Sci & Biotechnol Inst Bio21, Parkville, Vic, Australia

[3] Univ Calif San Diego, Dept Neurosurg, San Diego, CA 92103 USA

[4] Semmelweis Univ, Dept Genet Cell & Immunobiol, Budapest, Hungary

[5] Cedars Sinai Med Ctr, Los Angeles, CA 90048 USA

[6] Univ Oxford, Nuffield Dept Obstet & Gynaecol, Oxford, England

[7] Pohang Univ Sci & Technol, Dept Life Sci, Pohang, South Korea

[8] Bioinformat Inst, Singapore, Singapore

[9] La Trobe Univ, Dept Biochem, Melbourne, Vic, Australia

[10] Brown Univ, Warren Alpert Med Sch, Providence, RI 02912 USA

[11] Icahn Sch Med Mt Sinai, Cardiovasc Res Ctr, New York, NY 10029 USA

[12] Hiroshima Univ, Inst Biomed & Hlth Sci, Dept Cellular & Mol Biol, Hiroshima, Japan

[13] Univ Utrecht, Fac Vet Med, Dept Biochem & Cell Biol, Utrecht, Netherlands

[14] Johns Hopkins Sch Med, Dept Mol & Comparat Pathobiol, Baltimore, MD USA

[15] Inst Curie, INSERM U932, F-75005 Paris, France

来源：

JOURNAL OF EXTRACELLULAR VESICLES | 2014年 / 3卷 / 01期

关键词：

extracellular vesicles; microvesicles; microparticles; exosomes; ectosomes; extracellular RNA;

D O I：

10.3402/jev.v3.26913

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Secreted membrane-enclosed vesicles, collectively called extracellular vesicles (EVs), which include exosomes, ectosomes, microvesicles, microparticles, apoptotic bodies and other EV subsets, encompass a very rapidly growing scientific field in biology and medicine. Importantly, it is currently technically challenging to obtain a totally pure EV fraction free from non-vesicular components for functional studies, and therefore there is a need to establish guidelines for analyses of these vesicles and reporting of scientific studies on EV biology. Here, the International Society for Extracellular Vesicles (ISEV) provides researchers with a minimal set of biochemical, biophysical and functional standards that should be used to attribute any specific biological cargo or functions to EVs.

引用

页数：6

共 21 条

[1] Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma [J].

Arroyo, Jason D. ;

Chevillet, John R. ;

Kroh, Evan M. ;

Ruf, Ingrid K. ;

Pritchard, Colin C. ;

Gibson, Donald F. ;

Mitchell, Patrick S. ;

Bennett, Christopher F. ;

Pogosova-Agadjanyan, Era L. ;

Stirewalt, Derek L. ;

Tait, Jonathan F. ;

Tewari, Muneesh .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (12) :5003-5008

[2] Rab27a Supports Exosome-Dependent and -Independent Mechanisms That Modify the Tumor Microenvironment and Can Promote Tumor Progression [J].

Bobrie, Angelique ;

Krumeich, Sophie ;

Reyal, Fabien ;

Recchi, Chiara ;

Moita, Luis F. ;

Seabra, Miguel C. ;

Ostrowski, Matias ;

Thery, Clotilde .

CANCER RESEARCH, 2012, 72 (19) :4920-4930

[3] Intercellular Transport of MicroRNAs [J].

Boon, Reinier A. ;

Vickers, Kasey C. .

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2013, 33 (02) :186-192

[4] The influence of rotor type and centrifugation time on the yield and purity of extracellular vesicles [J].

Cvjetkovic, Aleksander ;

Lotvall, Jan ;

Lasser, Cecilia .

JOURNAL OF EXTRACELLULAR VESICLES, 2014, 3 (01)

[5] Large Oncosomes in Human Prostate Cancer Tissues and in the Circulation of Mice with Metastatic Disease [J].

Di Vizio, Dolores ;

Morello, Matteo ;

Dudley, Andrew C. ;

Schow, Peter W. ;

Adam, Rosalyn M. ;

Morley, Samantha ;

Mulholland, David ;

Rotinen, Mirja ;

Hager, Martin H. ;

Insabato, Luigi ;

Moses, Marsha A. ;

Demichelis, Francesca ;

Lisanti, Michael P. ;

Wu, Hong ;

Klagsbrun, Michael ;

Bhowmick, Neil A. ;

Rubin, Mark A. ;

D'Souza-Schorey, Crislyn ;

Freeman, Michael R. .

AMERICAN JOURNAL OF PATHOLOGY, 2012, 181 (05) :1573-1584

[6] Urokinase plasminogen activator and gelatinases are associated with membrane vesicles shed by human HT1080 fibrosarcoma cells [J].

Ginestra, A ;

Monea, S ;

Seghezzi, G ;

Dolo, V ;

Nagase, H ;

Mignatti, P ;

Vittorelli, ML .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (27) :17216-17222

[7] As we wait: coping with an imperfect nomenclature for extracellular vesicles [J].

Gould, Stephen J. ;

Raposo, Graca .

JOURNAL OF EXTRACELLULAR VESICLES, 2013, 2 (01)

[8] ISEV position paper: extracellular vesicle RNA analysis and bioinformatics [J].

Hill, Andrew F. ;

Pegtel, D. Michiel ;

Lambertz, Ulrike ;

Leonardi, Tommaso ;

O'Driscoll, Lorraine ;

Pluchino, Stefano ;

Ter-Ovanesyan, Dmitry ;

Nolte-'t Hoen, Esther N. M. .

JOURNAL OF EXTRACELLULAR VESICLES, 2013, 2 (01)

[9] Comparative proteomics evaluation of plasma exosome isolation techniques and assessment of the stability of exosomes in normal human blood plasma [J].

Kalra, Hina ;

Adda, Christopher G. ;

Liem, Michael ;

Ang, Ching-Seng ;

Mechler, Adam ;

Simpson, Richard J. ;

Hulett, Mark D. ;

Mathivanan, Suresh .

PROTEOMICS, 2013, 13 (22) :3354-3364

[10] Vesiclepedia: A Compendium for Extracellular Vesicles with Continuous Community Annotation [J].

Kalra, Hina ;

Simpson, Richard J. ;

Ji, Hong ;

Aikawa, Elena ;

Altevogt, Peter ;

Askenase, Philip ;

Bond, Vincent C. ;

Borras, Francesc E. ;

Breakefield, Xandra ;

Budnik, Vivian ;

Buzas, Edit ;

Camussi, Giovanni ;

Clayton, Aled ;

Cocucci, Emanuele ;

Falcon-Perez, Juan M. ;

Gabrielsson, Susanne ;

Gho, Yong Song ;

Gupta, Dwijendra ;

Harsha, H. C. ;

Hendrix, An ;

Hill, Andrew F. ;

Inal, Jameel M. ;

Jenster, Guido ;

Kraemer-Albers, Eva-Maria ;

Lim, Sai Kiang ;

Llorente, Alicia ;

Lotvall, Jan ;

Marcilla, Antonio ;

Mincheva-Nilsson, Lucia ;

Nazarenko, Irina ;

Nieuwland, Rienk ;

Nolte-'t Hoen, Esther N. M. ;

Pandey, Akhilesh ;

Patel, Tushar ;

Piper, Melissa G. ;

Pluchino, Stefano ;

Prasad, T. S. Keshava ;

Rajendran, Lawrence ;

Raposo, Graca ;

Record, Michel ;

Reid, Gavin E. ;

Sanchez-Madrid, Francisco ;

Schiffelers, Raymond M. ;

Siljander, Pia ;

Stensballe, Allan ;

Stoorvogel, Willem ;

Taylor, Douglas ;

Thery, Clotilde ;

Valadi, Hadi ;

van Balkom, Bas W. M. .

PLOS BIOLOGY, 2012, 10 (12)

← 1 2 3 →