IDENTIFICATION OF SURFACE-EXPOSED YERSINIA-PESTIS PROTEINS BY RADIO-IODINATION AND BIOTINYLATION

被引：5

作者：

ABATH, FGC ^{[1
]}

ALMEIDA, AMP ^{[1
]}

FERREIRA, LCS ^{[1
]}

机构：

[1] CTR PESQUISAS AGGEU,BR-50739 RECIFE,PE,BRAZIL

来源：

JOURNAL OF MEDICAL MICROBIOLOGY | 1992年 / 37卷 / 06期

关键词：

D O I：

10.1099/00222615-37-6-420

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

When whole cells (stationary phase) of Yersinia pestis strain EV76 were radiolabelled with Iodogen and I-136, 16 major and 10 minor surface-exposed outer membrane proteins (OMPs) were identified. Labelling with N-hydroxysuccinimidyl 6-biotinylamino-hexanoate (biotin X-NHS) resulted in a complex protein profile detectable after blotting and developing with peroxidase-conjugated avidin. Y. pestis cell fractionation revealed that biotin X-NHS labelled not only OMPs but also proteins of inner cell compartments. Therefore, radiolabelling was the more reliable technique for identifying the OMPs of Y. pestis.

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页码：420 / 424

页数：5

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