DETERMINATION OF ENDOTHELIN-CONVERTING ENZYME-ACTIVITY BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ONLINE RADIOACTIVE FLOW MONITORING

被引：5

作者：

HECKER, M ^{[1
]}

KAW, S ^{[1
]}

WALSH, DT ^{[1
]}

机构：

[1] ST BARTHOLOMEWS HOSP,MED CTR,WILLIAM HARVEY RES INST,LONDON EC1M 6BQ,ENGLAND

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 574卷 / 02期

关键词：

D O I：

10.1016/0378-4347(92)80050-Z

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid method to investigate the metabolism of I-125-labelled or non-labelled human big endothelin to endothelin-1 by reversed-phase high-performance liquid chromatography (HPLC) and on-line radioactive flow monitoring and/or ultraviolet detection was developed. Samples were processed by solid-phase extraction (average recovery 70-80% for non-labelled and 20-25% for I-125-labelled big endothelin and endothelin-1) followed by HPLC analysis (total analysis time 20 min). The method was successfully employed to monitor the conversion of big endothelin to endothelin-1 by various blood-borne cells, such as human polymorphonuclear leukocytes or monocytes/macrophage.

引用

页码：344 / 348

页数：5

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