TELOMERASE ACTIVITY IN GERMLINE AND EMBRYONIC-CELLS OF XENOPUS

被引：126

作者：

MANTELL, LL ^{[1
]}

GREIDER, CW ^{[1
]}

机构：

[1] COLD SPRING HARBOR LAB,COLD SPRING HARBOR,NY 11724

来源：

EMBO JOURNAL | 1994年 / 13卷 / 13期

关键词：

CELL CYCLE; EMBRYOGENESIS; GERMLINE; OOGENESIS; TELOMERASE;

D O I：

10.1002/j.1460-2075.1994.tb06620.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Telomerase is a ribonucleoprotein which synthesizes telomere repeats onto chromosome ends. Telomerase activity is involved in telomere length maimtenance. We used Xenopus laevis as a model system to study the expression of telomerase activity in germline cells and during early development. We identified a non-processive telomerase activity in manually dissected nuclei of Xenopus stage VI oocytes. Telomerase activity was detected throughout oogenesis and embryogenesis. Telomerase was active in both S and M phase cell cycle extracts, suggesting that telomerase activity is not regulated with chromosomal DNA replication.

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收藏

页码：3211 / 3217

页数：7

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