ORGANIZATION, PROMOTER ANALYSIS AND TRANSCRIPTIONAL REGULATION OF THE STAPHYLOCOCCUS-XYLOSUS XYLOSE UTILIZATION OPERON

被引：67

作者：

SIZEMORE, C

BUCHNER, E

RYGUS, T

WITKE, C

GOTZ, F

HILLEN, W

机构：

[1] UNIV ERLANGEN NURNBERG,LEHRSTUHL MIKROBIOL,STAUDSTR 5,W-8520 ERLANGEN,GERMANY

[2] UNIV TUBINGEN,W-7400 TUBINGEN 1,GERMANY

来源：

MOLECULAR & GENERAL GENETICS | 1991年 / 227卷 / 03期

关键词：

XYLOSE UTILIZATION; XYLOSE ISOMERASE; PROMOTER STRUCTURE; STAPHYLOCOCCI; XYL REGULON;

D O I：

10.1007/BF00273926

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Staphylococcus xylosus xyl genes were cloned in Staphylococcus carnosus by complementation to xylose utilization. Xylose isomerase assays under inducing (xylose present) and non-inducing (xylose absent) conditions indicated the presence of a regulated xylA gene on the recombinant plasmid. The nucleotide sequence (4520 bases) revealed three open reading frames with the same polarity. They were identified by sequence homologies as xylR, encoding the Xyl repressor, xylA, encoding xylose isomerase and xylB, encoding xylulokinase. Primer extension analyses indicated constitutive transcription of xylR and xylose-inducible transcription of xylA. Promoter consensus sequences were found up-stream of both transcriptional start sites. A transcriptional terminator between xylR and xylA separates the different transcriptional units. Potential regulatory elements were identified by sequence analysis and suggest a repressor-operator mechanism for the regulation of xylAB expression.

引用

页码：377 / 384

页数：8

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[11] PREPARATION OF MILLIGRAM AMOUNTS OF 21-DEOXYRIBONUCLEIC ACID RESTRICTION FRAGMENTS [J].