INTRINSIC TRANSCRIPT CLEAVAGE ACTIVITY OF RNA-POLYMERASE

被引:185
作者
ORLOVA, M
NEWLANDS, J
DAS, A
GOLDFARB, A
BORUKHOV, S
机构
[1] PUBL HLTH RES INST,NEW YORK,NY 10016
[2] UNIV CONNECTICUT,CTR HLTH,FARMINGTON,CT 06030
关键词
D O I
10.1073/pnas.92.10.4596
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The GreA and GreB transcript cleavage factors of Escherichia coli suppress elongation arrest and may have a proofreading role in transcription. With the use off. coli greA(-)greB(-) mutant, RNA polymerase is demonstrated to possess substantial intrinsic transcript cleavage activity. Mildly alkaline pH mimics the effect of the Gre proteins by inducing transcript cleavage in ternary complexes and antagonizing elongation arrest through a cleavage-and-restart reaction. Thus, transcript cleavage constitutes the second enzymological activity of RNA polymerase along with polymerization/pyrophosphorolysis of RNA, whereas the Gre proteins merely enhance this intrinsic property.
引用
收藏
页码:4596 / 4600
页数:5
相关论文
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