METABOLISM OF EXOGENOUSLY APPLIED FRUCTOSE-1,6-BISPHOSPHATE IN HYPOXIC VASCULAR SMOOTH-MUSCLE

被引:53
作者
HARDIN, CD
ROBERTS, TM
机构
来源
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | 1994年 / 267卷 / 06期
关键词
C-13 NUCLEAR MAGNETIC RESONANCE; GLYCOLYSIS; HOG CAROTID ARTERY; VASCULAR TONE; ISOMETRIC FORCE; ISCHEMIA;
D O I
10.1152/ajpheart.1994.267.6.H2325
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Exogenously administered fructose 1,6-bisphosphate reportedly protects ischemic or hypoxic tissue and facilitates metabolic recovery. The mechanism of action of exogenous fructose 1,6-bisphosphate has been an issue of considerable debate, since there is a lack of direct evidence that fructose 1,6-bisphosphate can cross the cell membrane and act as an intermediate in glycolysis. We synthesized [1,6-C-13]fructose 1,6-bisphosphate and directly examined its cellular metabolism in hog carotid artery segments using C-13-nuclear magnetic resonance (NMR) spectroscopy. [1,6-C-13]fructose 1,6-bisphosphate (2.1 mM) was metabolized by hog carotid artery during normoxia and hypoxia with a major metabolic product being [3-C-13]lactate. The production of [3-C-13]lactate was greater during hypoxia than during normoxia, indicating that fructose 1,6-bisphosphate metabolism responded to the energetic state of the tissue. We found that exogenously added fructose 1,6-bisphosphate at 2.1 mM did not significantly improve the ability of hypoxic hog carotid artery to maintain isometric force, whereas 20 mM fructose 1,6-bisphosphate did significantly, although modestly, improve isometric force maintenance. These results indicate that exogenously added fructose 1,6-bisphosphate is capable of entering cells and serving as a glycolytic intermediate.
引用
收藏
页码:H2325 / H2332
页数:8
相关论文
共 22 条
[11]   SIMULTANEOUS AND SEPARABLE FLUX OF PATHWAYS FOR GLUCOSE AND GLYCOGEN UTILIZATION STUDIED BY C-13-NMR [J].
HARDIN, CD ;
KUSHMERICK, MJ .
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 1994, 26 (09) :1197-1210
[12]   TAUTOMERIC COMPOSITION OF D-FRUCTOSE PHOSPHATES IN SOLUTION BY FOURIER-TRANSFORM C-13 NUCLEAR MAGNETIC-RESONANCE [J].
KOERNER, TAW ;
CARY, LW ;
BHACCA, NS ;
YOUNATHAN, ES .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1973, 51 (03) :543-550
[13]   PRESERVING EFFECT OF FRUCTOSE-1,6-BISPHOSPHATE ON HIGH-ENERGY PHOSPHATE-COMPOUNDS DURING ANOXIA AND REPERFUSION IN ISOLATED LANGENDORFF-PERFUSED RAT HEARTS [J].
LAZZARINO, G ;
NUUTINEN, ME ;
TAVAZZI, B ;
CERRONI, L ;
DIPIERRO, D ;
GIARDINA, B .
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 1991, 23 (01) :13-23
[14]   INCREASE OF INTRAERYTHROCYTIC FRUCTOSE-1,6-DIPHOSPHATE AFTER INCUBATION OF WHOLE HUMAN-BLOOD WITH FRUCTOSE-1,6-DIPHOSPHATE [J].
LAZZARINO, G ;
CATTANI, L ;
COSTRINI, R ;
MULIERI, L ;
CANDIANI, A ;
GALZIGNA, L .
CLINICAL BIOCHEMISTRY, 1984, 17 (01) :42-45
[15]  
Lowry OH, 1972, FLEXIBLE SYSTEM ENZY
[16]   COMPARTMENTATION OF GLYCOLYTIC AND GLYCOGENOLYTIC METABOLISM IN VASCULAR SMOOTH-MUSCLE [J].
LYNCH, RM ;
PAUL, RJ .
SCIENCE, 1983, 222 (4630) :1344-1346
[17]   HEMODYNAMIC, ELECTROCARDIOGRAPHIC, AND METABOLIC EFFECTS OF FRUCTOSE DIPHOSPHATE ON ACUTE MYOCARDIAL ISCHEMIA [J].
MARKOV, AK ;
OGLETHORPE, NC ;
BLAKE, TM ;
LEHAN, PH ;
HELLEMS, HK .
AMERICAN HEART JOURNAL, 1980, 100 (05) :639-646
[18]   PREFERENTIAL SUPPORT OF CA-2+ UPTAKE IN SMOOTH-MUSCLE PLASMA-MEMBRANE VESICLES BY AN ENDOGENOUS GLYCOLYTIC CASCADE [J].
PAUL, RJ ;
HARDIN, CD ;
RAEYMAEKERS, L ;
WUYTACK, F ;
CASTEELS, R .
FASEB JOURNAL, 1989, 3 (11) :2298-2301
[19]  
PAUL RJ, 1980, HDB PHYSL 2, V2, P201
[20]   MECHANISMS OF GLYCOLYTIC INHIBITION IN ISCHEMIC RAT HEARTS [J].
ROVETTO, MJ ;
LAMBERTON, WF ;
NEELY, JR .
CIRCULATION RESEARCH, 1975, 37 (06) :742-751