CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF A LIPASE FROM BACILLUS-SUBTILIS

被引：9

作者：

RANSAC, S

BLAAUW, M

LESUISSE, E

SCHANCK, K

COLSON, C

DIJKSTRA, BW

机构：

[1] UNIV GRONINGEN,BIOPHYS CHEM LAB,9747 AG GRONINGEN,NETHERLANDS

[2] UNIV GRONINGEN,BIOSON RES INST,DEPT CHEM,9747 AG GRONINGEN,NETHERLANDS

[3] UNIV CATHOLIQUE LOUVAIN,UNITE GENET,GENET MICROBIENNE LAB,B-1348 LOUVAIN,BELGIUM

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1994年 / 238卷 / 05期

关键词：

CRYSTAL; X-RAY; LIPASE; ESTERASE; BACILLUS;

D O I：

10.1006/jmbi.1994.1342

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Single crystals of the lipase from Bacillus subtilis have been obtained using a mixture of polyethylene glycol 4000 and sodium sulphate solution as the precipitant. The crystals grow at room temperature in two to three weeks in the presence of n-octyl-β-D-glucoside. They belong to the monoclinic space group C2 with a = 121·20 Å, b = 93·19 Å, c = 80·96 Å, and β = 110·67°, with four protein molecules per asymmetric unit. The crystals diffract to at least 2·5 Å resolution and are suitable for an X-ray structure analysis. © 1994 Academic Press Limited.

引用

页码：857 / 859

页数：3

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