QUANTIFICATION OF CYCLIC 2,3-DIPHOSPHOGLYCERATE FROM METHANOGENIC BACTERIA BY ISOTACHOPHORESIS

被引：14

作者：

GORRIS, LGM ^{[1
]}

KORTELAND, J ^{[1
]}

DERKSEN, RJAM ^{[1
]}

VANDERDRIFT, C ^{[1
]}

VOGELS, GD ^{[1
]}

机构：

[1] CATHOLIC UNIV NIJMEGEN,FAC SCI,DEPT MICROBIOL,6525 ED NIJMEGEN,NETHERLANDS

来源：

JOURNAL OF CHROMATOGRAPHY | 1990年 / 504卷 / 02期

关键词：

D O I：

10.1016/S0021-9673(01)89545-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Cyclic 2,3-diphosphoglycerate (cDPG), a metabolite exclusively present in methanogenic bacteria, was separated from other phosphate-containing compounds in extracts of Methanobacterium thermoautotrophicum by means of isotachophoresis. The detection limit was 100 pmol per injected sample. Quantification of intracellular concentrations of cDPG with the isotachophoretic assay and with a spectrophoto-metric cDPG assay gave comparable results, but the isotachophoretic assay was faster, less laborious and more sensitive. A possible involvement of cDPG in the energy metabolism was indicated by studies of Mb. thermoautotrophicum in batch and continuous cultures. © 1990.

引用

页码：421 / 428

页数：8

共 13 条

[1] EVANS JNS, 1986, J BIOL CHEM, V261, P6323
[2] Everaerts FM, 1976, J CHROMATOGRAPHY LIB, V6
[3] GORRIS LGM, UNPUB
[4] THERMOADAPTATION OF METHANOGENIC BACTERIA BY INTRACELLULAR ION CONCENTRATION
HENSEL, R
KONIG, H
[J]. FEMS MICROBIOLOGY LETTERS, 1988, 49 (01) : 75 - 79
[5] ANALYSIS OF COENZYME-M (2-MERCAPTOETHANESULFONIC ACID) DERIVATIVES BY ISOTACHOPHORESIS
HERMANS, JMH
HUTTEN, TJ
VANDERDRIFT, C
VOGELS, GD
[J]. ANALYTICAL BIOCHEMISTRY, 1980, 106 (02) : 363 - 366
[6] COENZYME M DERIVATIVES AND THEIR EFFECTS ON METHANE FORMATION FROM CARBON-DIOXIDE AND METHANOL BY CELL-EXTRACTS OF METHANOSARCINA-BARKERI
HUTTEN, TJ
DEJONG, MH
PEETERS, BPH
VANDERDRIFT, C
VOGELS, GD
[J]. JOURNAL OF BACTERIOLOGY, 1981, 145 (01) : 27 - 34
[7] METHANOPHOSPHAGEN - UNIQUE CYCLIC PYROPHOSPHATE ISOLATED FROM METHANOBACTERIUM-THERMOAUTOTROPHICUM
KANODIA, S
ROBERTS, MF
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (17): : 5217 - 5221
[8] ELECTRON-TRANSFER REACTIONS IN METHANOGENS
KELTJENS, JT
VANDERDRIFT, C
[J]. FEMS MICROBIOLOGY LETTERS, 1986, 39 (03) : 259 - 303
[9] ATP SYNTHESIS DRIVEN BY A POTASSIUM DIFFUSION POTENTIAL IN METHANOBACTERIUM-THERMOAUTOTROPHICUM IS STIMULATED BY SODIUM
SCHONHEIT, P
PERSKI, HJ
[J]. FEMS MICROBIOLOGY LETTERS, 1983, 20 (02) : 263 - 267
[10] THE CYCLIC-2,3-DIPHOSPHOGLYCERATE FROM METHANOBACTERIUM-THERMOAUTOTROPHICUM IS THE D-ENANTIOMER
SEELY, RJ
FAHRNEY, DE
[J]. CURRENT MICROBIOLOGY, 1984, 10 (02) : 85 - 87

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