QUANTIFICATION OF CYCLIC 2,3-DIPHOSPHOGLYCERATE FROM METHANOGENIC BACTERIA BY ISOTACHOPHORESIS

被引：14

作者：

GORRIS, LGM ^{[1
]}

KORTELAND, J ^{[1
]}

DERKSEN, RJAM ^{[1
]}

VANDERDRIFT, C ^{[1
]}

VOGELS, GD ^{[1
]}

机构：

[1] CATHOLIC UNIV NIJMEGEN,FAC SCI,DEPT MICROBIOL,6525 ED NIJMEGEN,NETHERLANDS

来源：

JOURNAL OF CHROMATOGRAPHY | 1990年 / 504卷 / 02期

关键词：

D O I：

10.1016/S0021-9673(01)89545-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Cyclic 2,3-diphosphoglycerate (cDPG), a metabolite exclusively present in methanogenic bacteria, was separated from other phosphate-containing compounds in extracts of Methanobacterium thermoautotrophicum by means of isotachophoresis. The detection limit was 100 pmol per injected sample. Quantification of intracellular concentrations of cDPG with the isotachophoretic assay and with a spectrophoto-metric cDPG assay gave comparable results, but the isotachophoretic assay was faster, less laborious and more sensitive. A possible involvement of cDPG in the energy metabolism was indicated by studies of Mb. thermoautotrophicum in batch and continuous cultures. © 1990.

引用

页码：421 / 428

页数：8