GENE-TRANSFER BY JET INJECTION INTO DIFFERENTIATED TISSUES OF LIVING ANIMALS AND IN ORGAN-CULTURE

被引：20

作者：

FURTH, PA

KERR, D

WALL, R

机构：

[1] UNIV MARYLAND,SCH MED,DEPT MED,DIV INFECT DIS,BALTIMORE,MD 21201

[2] VET ADM MED CTR,BALTIMORE,MD 21201

[3] USDA ARS,GENE EVALUAT & MAPPING LAB,BELTSVILLE,MD

[4] ALTRABIO INC,ARDEN HILLS,MN

来源：

MOLECULAR BIOTECHNOLOGY | 1995年 / 4卷 / 02期

关键词：

JET INJECTION; GENE TRANSFER; GENE THERAPY; DNA IMMUNIZATION;

D O I：

10.1007/BF02921606

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Jet injection can be used to introduce genes into the cells of differentiated tissues of living animals and organ cultures. When a solution of plasmid DNA is jet injected into a selected tissue or organ, cells lying in or near the path of the jet injection are transfected with DNA and the introduced gene(s) are expressed. Since there is minimal morbidity from each jet injection, multiple injections can be performed at the same or nearby sites. Both mRNA and protein expression from transfected genes can be quantitated using standard methods. In addition, the technique is an efficient means of DNA immunization. Methodology for using jet injection to transfer plasmid DNA into the cells of skin, fat, mammary gland, and muscle are described.

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页码：121 / 127

页数：7

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