Elevated calcium level induces calcium-dependent proteolysis of A-CAM (N-cadherin) in heart - Analysis by detergent-treated model

被引：15

作者：

Sato, N ^{[1
]}

Fujio, Y ^{[1
]}

YamadaHonda, F ^{[1
]}

Funai, H ^{[1
]}

Wada, A ^{[1
]}

Kawashima, S ^{[1
]}

Awata, N ^{[1
]}

Shibata, N ^{[1
]}

机构：

[1] TOKYO METROPOLITAN INST MED SCI,DEPT MOLEC BIOL,BUNKYO KU,TOKYO 113,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 217卷 / 02期

关键词：

D O I：

10.1006/bbrc.1995.2823

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Calcium overload induces cardiac muscle cell dysfunction in cardiovascular diseases. We investigated the effects of elevated calcium level on adherens-junction-specific cell adhesion molecule (A-CAM). Incubation of Triton X-100-treated canine heart homogenate in the presence of Ca2+ reduced the content of A-CAM. Reduction in A-CAM requires milli-molar Ca2+ and was inhibited by protease inhibitors, leupeptin and calpeptin. Immunohistochemical observation revealed that m-calcium-activated neutral protease (m-CANP) was colocalized with A-CAM in intercalated disks. These data suggested that m-CANP proteolyzes A-CAM in response to calcium overload in cardiac muscle. (C) 1995 Academic Press, Inc.

引用

页码：649 / 653

页数：5

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