MODULATION OF GENE-EXPRESSION IN THE PREIMPLANTATION MOUSE EMBRYO BY TGF-ALPHA AND TGF-BETA

被引：40

作者：

BABALOLA, GO ^{[1
]}

SCHULTZ, RM ^{[1
]}

机构：

[1] UNIV PENN, DEPT BIOL, PHILADELPHIA, PA 19104 USA

来源：

MOLECULAR REPRODUCTION AND DEVELOPMENT | 1995年 / 41卷 / 02期

关键词：

PREIMPLANTATION MOUSE EMBRYO; GENE EXPRESSION; MESSENGER-RNA DIFFERENTIAL DISPLAY; TGF-ALPHA; TGF-BETA;

D O I：

10.1002/mrd.1080410203

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of growth factors on regulating gene expression in the preimplantation mouse embryo was examined, since results of previous experiments revealed a stimulatory effect of exogenously-added growth factors on preimplantation development in vitro. Treatment of early cavitating blastocysts with either 250 pM TGF-alpha or TGF-beta results in changes in the pattern of total protein synthesis as assessed by high-resolution two-dimensional gel electrophoresis. In some cases, the synthesis of a particular polypeptide is either up- or downregulated by each growth factor, whereas in other instances the synthesis of a polypeptide is modulated by one but not the other growth factor. Use of the mRNA differential display method permitted the identification of genes whose expression is either up- or downregulated by these growth factors. Treatment of mouse blastocysts with either TGF-alpha or TGF-beta results in the increased expression of the b subunit of the F-0 ATPase. TGF-beta also stimulates the expression of the DNA polymerase alpha. TGF-alpha treatment results in the increase in expression of a gene homologous to the human HEPG2 cDNA, as well as in a decrease in expression of fibronectin. (C) 1995 Wiley-Liss, Inc.

引用

页码：133 / 139

页数：7

共 40 条

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