MODULATION OF GENE-EXPRESSION IN THE PREIMPLANTATION MOUSE EMBRYO BY TGF-ALPHA AND TGF-BETA

被引：40

作者：

BABALOLA, GO ^{[1
]}

SCHULTZ, RM ^{[1
]}

机构：

[1] UNIV PENN, DEPT BIOL, PHILADELPHIA, PA 19104 USA

来源：

MOLECULAR REPRODUCTION AND DEVELOPMENT | 1995年 / 41卷 / 02期

关键词：

PREIMPLANTATION MOUSE EMBRYO; GENE EXPRESSION; MESSENGER-RNA DIFFERENTIAL DISPLAY; TGF-ALPHA; TGF-BETA;

D O I：

10.1002/mrd.1080410203

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of growth factors on regulating gene expression in the preimplantation mouse embryo was examined, since results of previous experiments revealed a stimulatory effect of exogenously-added growth factors on preimplantation development in vitro. Treatment of early cavitating blastocysts with either 250 pM TGF-alpha or TGF-beta results in changes in the pattern of total protein synthesis as assessed by high-resolution two-dimensional gel electrophoresis. In some cases, the synthesis of a particular polypeptide is either up- or downregulated by each growth factor, whereas in other instances the synthesis of a polypeptide is modulated by one but not the other growth factor. Use of the mRNA differential display method permitted the identification of genes whose expression is either up- or downregulated by these growth factors. Treatment of mouse blastocysts with either TGF-alpha or TGF-beta results in the increased expression of the b subunit of the F-0 ATPase. TGF-beta also stimulates the expression of the DNA polymerase alpha. TGF-alpha treatment results in the increase in expression of a gene homologous to the human HEPG2 cDNA, as well as in a decrease in expression of fibronectin. (C) 1995 Wiley-Liss, Inc.

引用

页码：133 / 139

页数：7

共 40 条

[31] Shi CZ, Collings HW, Buettger CW, Garside WT, Matschinsky FM, Heyner S, Insulin family growth factors have specific effects on protein synthesis in preimplantation mouse embryos, Mol Reprod Dev, 37, pp. 398-406, (1994)
[32] Sporn MB, Roberts AB, Peptide growth factors are multifunctional, Nature, 332, pp. 217-219, (1988)
[33] Temeles GL, Ram PT, Rothstein JL, Schultz RM, Expression patterns of novel genes during mouse preimplantation embryogenesis, Mol Reprod Dev, 37, pp. 121-129, (1994)
[34] Treisman R, Ternary complex factors: growth factor‐regulated transcriptional activators, Current Opinion in Genetics & Development, 4, pp. 96-101, (1994)
[35] Wahl AF, Geis AM, Spain BH, Wong SW, Korn D, Wang TSF, Gene expression of human DNA polymerase alpha during cell proliferation and the cell cycle, Mol Cell Biol, 8, pp. 5016-5025, (1988)
[36] Wartiovaara J, Leivo I, Vaheri A, Expression of the cell surface‐associated glycoprotein, fibronectin, in the early mouse embryo, Dev Biol, 69, pp. 247-257, (1979)
[37] Werb Z, Expression of EGF and TGF‐α genes in early mammalian development, Mol Reprod Dev, 27, pp. 10-15, (1990)
[38] Whitten WK, Nutrient requirements for the culture of preimplantation mouse embryos in vitro, Adv Biosci, 6, pp. 129-139, (1971)
[39] Wong SW, Wahl AF, Yuan P-M, Arai N, Pearson BE, Arai K, Korn D, Hunkapiller MW, Wang TSF, Human DNA polymerase α gene expression is cell proliferation dependent and its primary structure is similar to both procaryotic and eucaryotic replicative DNA polymerases, EMBO J, 7, pp. 37-47, (1988)
[40] Zimmermann JW, Schultz RM, Analysis of gene expression in the preimplantation mouse embryo: use of mRNA differential display, Proc Natl Acad Sci USA, 91, pp. 5456-5460, (1994)

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