THYRSIFERYL 23-ACETATE IS A NOVEL SPECIFIC INHIBITOR OF PROTEIN PHOSPHATASE PP2A

被引:42
作者
MATSUZAWA, S
SUZUKI, T
SUZUKI, M
MATSUDA, A
KAWAMURA, T
MIZUNO, Y
KIKUCHI, K
机构
[1] HOKKAIDO UNIV, CHEM LAB, KUSHIRO 085, JAPAN
[2] HOKKAIDO UNIV, GRAD SCH ENVIRONM EARTH SCI, KITA KU, SAPPORO, HOKKAIDO 060, JAPAN
来源
FEBS LETTERS | 1994年 / 356卷 / 2-3期
关键词
THYRSIFERYL; 23-ACETATE; PROTEIN PHOSPHATASE 2A;
D O I
10.1016/0014-5793(94)01281-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Thyrsiferyl 23-acetate (TF23A), a cytotoxic compound from marine red alga, has been shown to potently and specifically inhibit serine/threonine protein phosphatase 2A (PP2A) with IC50 values of 4-16 mu M, depending on the enzyme concentration. TF23A did not affect activity of protein phosphatase 1 (PP1), 2B (PP2B), 2C (PP2C), or protein tyrosine phosphatases (PTP) up to 1 mM. It inhibited PP2A activity in a crude extract of a human T cell line, Jurkat cell, as well as the purified catalytic subunit. Thus, TF23A proved to be a novel useful probe for clearly distinguishing the activity of PP2A from those of the other protein phosphatases in crude cell extracts and identification of cellular processes that are regulated by PP2A.
引用
收藏
页码:272 / 274
页数:3
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