IMMOBILIZATION AND CHARACTERIZATION OF PHENYLETHANOL DEHYDROGENASE FROM LACTOBACILLUS KEFIR AND ITS APPLICATION IN A FLOW-INJECTION-ANALYSIS SYSTEM

被引：4

作者：

TARHAN, L ^{[1
]}

HUMMEL, W ^{[1
]}

机构：

[1] HEINRICH HEINE UNIV, FORSCHUNGSZENTRUM JULICH, INST ENZYMTECNOL, W-5170 JULICH, GERMANY

来源：

PROCESS BIOCHEMISTRY | 1995年 / 30卷 / 01期

关键词：

D O I：

10.1016/0032-9592(95)95704-M

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phenylethanol dehydrogenase was isolated from Lactobacillus kefir, and some kinetic parameters and properties of native and immobilised phenylethanol dehydrogenase on the modified DEAE-Cellulose, Bioran, Mobitec, and VA-epoxy were investigated. The activity of immobilised phenylethanol dehydrogenase was examined in a glow-injection-analysis system in relation to the concentration of diacetyl in water and beer.

引用

页码：49 / 55

页数：7

共 11 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] LACTOBACILLUS-KEFIR ALCOHOL-DEHYDROGENASE - A USEFUL CATALYST FOR SYNTHESIS [J].

BRADSHAW, CW ;

HUMMEL, W ;

WONG, CH .

JOURNAL OF ORGANIC CHEMISTRY, 1992, 57 (05) :1532-1536

[3]

DE MAN J. C., 1960, JOUR APPL BACT, V23, P130, DOI 10.1111/j.1365-2672.1960.tb00188.x

[4]

Dixon M., 1979, ENZYMES, P47

[5]

HUMMEL W, 1990, APPL MICROBIOL BIOT, V34, P15, DOI 10.1007/BF00170916

[6] ENZYME-CATALYZED SYNTHESIS OF OPTICALLY PURE R(+)-PHENYLETHANOL [J].

HUMMEL, W .

BIOTECHNOLOGY LETTERS, 1990, 12 (06) :403-408

[7]

HUMMEL W, 1989, BIOCATALYSIS, V2, P293

[8] EFFECT OF ENZYME RATIO ON THE PROPERTIES OF GLUCOSE-OXIDASE AND CATALASE IMMOBILIZED ON MODIFIED PERLITE [J].

TARHAN, L ;

TELEFONCU, A .

PROCESS BIOCHEMISTRY, 1992, 27 (01) :11-15

[9]

TARHAN L, 1990, BIOMED BIOCHIM ACTA, V49, P307

[10]

ZABORSKY CR, 1973, IMMOBILIZED ENZYMES, P49

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