A SIMPLE IMPRINT-HYBRIDIZATION METHOD FOR DETECTION OF VIROIDS

被引：22

作者：

ROMERODURBAN, J ^{[1
]}

CAMBRA, M ^{[1
]}

DURANVILA, N ^{[1
]}

机构：

[1] INST VALENCIANO INVEST AGR,DEPT PROTECC VEGETAL & BIOTECNOL,E-46113 MONCADA,SPAIN

来源：

JOURNAL OF VIROLOGICAL METHODS | 1995年 / 55卷 / 01期

关键词：

IMPRINT-HYBRIDIZATION; VIROID; INDEXING; COMMERCIAL CROP;

D O I：

10.1016/0166-0934(95)00043-T

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An imprint-hybridization method has been designed to simplify the processing of samples during routine viroid indexing. The method requires minimal sample manipulation and has been evaluated for detection of viroids in 11 viroid-host combinations including 4 viroids (CEVd, CSVd, HSVd, ASBVd) and 7 hosts (chrysanthemum, citron, cucumber, Gynura, tomato, peach and avocado). The method is fast and sensitive, and provides additional information on the sites of viroid accumulation.

引用

页码：37 / 47

页数：11

共 17 条

[1] DETECTION OF DOUBLE-STRANDED-RNA BY ELISA AND DOT IMMUNOBINDING ASSAY USING AN ANTISERUM TO SYNTHETIC POLYNUCLEOTIDES [J].

ARAMBURU, J ;

NAVASCASTILLO, J ;

MORENO, P ;

CAMBRA, M .

JOURNAL OF VIROLOGICAL METHODS, 1991, 33 (1-2) :1-11

[2] P-32 LABELED AND BIOTIN-LABELED INVITRO TRANSCRIBED CRNA PROBES FOR THE DETECTION OF POTATO SPINDLE TUBER VIROID AND CHRYSANTHEMUM STUNT VIROID [J].

CANDRESSE, T ;

MACQUAIRE, G ;

BRAULT, V ;

MONSION, M ;

DUNEZ, J .

RESEARCH IN VIROLOGY, 1990, 141 (01) :97-107

[3] A DEFINITION OF CITRUS VIROID GROUPS AND THEIR RELATIONSHIP TO THE EXOCORTIS DISEASE [J].

DURANVILA, N ;

ROISTACHER, CN ;

RIVERABUSTAMANTE, R ;

SEMANCIK, JS .

JOURNAL OF GENERAL VIROLOGY, 1988, 69 :3069-3080

[4]

FRANCIS M, 1993, THESIS U CALIFORNIA

[5]

GARCIALUQUE I, 1986, VIROLOGY, V48, P275

[6]

GARNSEY SM, 1993, 12TH P C IOCV RIV, P39

[7] DIRECT TISSUE BLOTTING FOR DETECTION OF TOMATO SPOTTED WILT VIRUS IN IMPATIENS [J].

HSU, HT ;

LAWSON, RH .

PLANT DISEASE, 1991, 75 (03) :292-295

[8]

Jensen W.A., 1962, BOT HISTOCHEMISTRY P

[9] IMMUNOLOGICAL DETECTION OF PLANT-VIRUSES AND A MYCOPLASMALIKE ORGANISM BY DIRECT TISSUE BLOTTING ON NITROCELLULOSE MEMBRANES [J].

LIN, NS ;

HSU, YH ;

HSU, HT .

PHYTOPATHOLOGY, 1990, 80 (09) :824-828

[10]

Maniatis T, 1989, MOL CLONING

← 1 2 →