ACTIVITIES OF HUMAN ACIDIC FIBROBLAST GROWTH-FACTOR IN AN INVITRO DERMAL EQUIVALENT MODEL

被引:23
作者
DUBERTRET, L
BRUNNERFERBER, F
MISITI, J
THOMAS, KA
DUBERTRET, ML
机构
[1] HOP HENRI MONDOR, INSERM, U312, F-94010 CRETEIL, FRANCE
[2] HOP HENRI MONDOR, ARIC, F-94010 CRETEIL, FRANCE
[3] MERCK SHARP & DOHME LTD, DEPT CLIN PHARMACOL, RAHWAY, NJ 07065 USA
[4] MERCK SHARP & DOHME LTD, DEPT BIOCHEM, RAHWAY, NJ 07065 USA
关键词
D O I
10.1111/1523-1747.ep12486796
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Acidic fibroblast growth factor is a potent mitogen for human dermal fibroblasts in an in vitro three-dimensional collagen matrix, the "dermal equivalent." Both cell numbers and DNA synthesis are optimally stimulated by daily doses of 1 ng/ml of the pure human mitogen in the presence of heparin, which binds to, and stabilizes, the protein. Under daily treatment by 1 ng/ml aFGF, the fibroblast mitogenic response is marked but transient, and decreases steadily when fibroblasts mature in the collagen matrix. aFGF mitogenic stimulation also results in a decrease in cellular volume and inhibition of fibroblast-mediated contraction of the collagen gel. Various dosing regimes indicate that, although the greatest mitotic response was generated by daily dosing, nearly optimal responses can also be achieved with either a short duration of early daily dosing or longer-term intermittent treatment.
引用
收藏
页码:793 / 798
页数:6
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