The Tomato yellow leaf curl virus (TYLCV) V2 protein interacts with the host papain-like cysteine protease CYP1

被引:33
作者
Bar-Ziv, Amalia [1 ,2 ]
Levy, Yael [1 ]
Hak, Hagit [1 ,3 ]
Mett, Anahit [1 ]
Belausov, Eduard [1 ]
Citovsky, Vitaly [4 ]
Gafni, Yedidya [1 ,2 ]
机构
[1] Volcani Ctr, Inst Plant Sci, ARO, Bet Dagan, Israel
[2] Bar Ilan Univ, Mina & Everard Goodman Fac Life Sci, Ramat Gan, Israel
[3] Hebrew Univ Jerusalem, Alexander Silberman Inst Life Sci, Dept Biol Chem, Jerusalem, Israel
[4] SUNY Stony Brook, Dept Biochem & Cell Biol, Stony Brook, NY 11794 USA
关键词
TYLCV; V2; protein; papain-like cysteine protease; CYP1; protein interaction;
D O I
10.4161/psb.20935
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The V2 protein of Tomato yellow leaf curl geminivirus ( TYLCV) is an RNA- silencing suppressor that counteracts the innate immune response of the host plant. however, this anti-host defense function of V2 may include targeting of other defensive mechanisms of the plant. Specifically, we show that V2 recognizes and directly binds the tomato CYP1 protein, a member of the family of papain-like cysteine proteases which are involved in plant defense against diverse pathogens. this binding occurred both in vitro and in vivo, within living plant cells. the V2 binding site within mCYP1 was identified in the direct proximity to the papain-like cysteine protease active site.
引用
收藏
页码:983 / 989
页数:7
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