ADRENALECTOMY REDUCES ALPHA-1 AND NOT BETA-1 NA+-K+-ATPASE MESSENGER-RNA EXPRESSION IN RAT DISTAL NEPHRON

被引:45
作者
FARMAN, N
COUTRY, N
LOGVINENKO, N
BLOTCHABAUD, M
BOURBOUZE, R
BONVALET, JP
机构
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1992年 / 263卷 / 04期
关键词
INSITU HYBRIDIZATION; ALPHA-2-ISOFORMS; ALPHA-3-ISOFORMS AND BETA-2-ISOFORMS; CORTICOSTEROIDS;
D O I
10.1152/ajpcell.1992.263.4.C810
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
The abundance of mRNA of alpha1-, alpha2-, alpha3-, beta1-, and beta2-isoforms of Na+-K+-ATPase was examined in several renal structures of normal and adrenalectomized (ADX) rats. In situ hybridization with S-35-labeled cRNA probes was performed on kidney sections from adult rats. The number of silver grains per unit surface area was quantified over cells of the glomerulus, proximal convoluted tubules (PCT), early distal tubules (EDT), and cortical collecting ducts (CCD). In normal rat kidney, alpha1- and beta1-mRNA was detected in PCT, EDT, and CCD, with the following range of magnitude: EDT > CCD > PCT > glomerulus. The amount of alpha1- and beta1-mRNA was equivalent. A large abundance of these two mRNA species was also found in the medullary thick ascending limb of the loop of Henle. Expression of alpha2, alpha3, and beta2 was very low and evenly distributed over any cell type. In ADX, a significant decrease in alpha1-mRNA (30%) was observed in EDT and CCD, with no change in PCT. Beta1-mRNA abundance was unaffected by adrenalectomy. These results indicate that 1) in the rat kidney alpha1- and beta1-mRNA are coexpressed at a similar level that varies along the renal tubule according to the cell type, 2) minute expression of alpha2-, alpha3-, and beta2-mRNA is present in the kidney, and 3) corticosteroid depletion reduces the expression of alpha1- and not beta1-mRNA in the corticosteroid-sensitive tubular cells.
引用
收藏
页码:C810 / C817
页数:8
相关论文
共 33 条
  • [1] IMMUNODETECTION OF NA,K-ATPASE ALPHA-3-ISOFORM IN RENAL AND NERVE TISSUES
    ARYSTARKHOVA, EA
    LAKHTINA, OE
    MODYANOV, NN
    [J]. FEBS LETTERS, 1989, 250 (02) : 545 - 548
  • [2] DIFFERENCE IN THE NA AFFINITY OF NA+-K+-ATPASE ALONG THE RABBIT NEPHRON - MODULATION BY K
    BARLETBAS, C
    CHEVAL, L
    KHADOURI, C
    MARSY, S
    DOUCET, A
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1990, 259 (02): : F246 - F250
  • [3] BHUTADA A, 1991, J BIOL CHEM, V266, P10859
  • [4] BLOTCHABAUD M, 1990, J BIOL CHEM, V265, P11676
  • [5] EFFECT OF CELL SODIUM ON NA+/K+-ATPASE-DEPENDENT SODIUM-EFFLUX IN CORTICAL COLLECTING TUBULE OF RABBITS UNDER DIFFERENT ALDOSTERONE STATUS
    BLOTCHABAUD, M
    JAISSER, F
    BONVALET, JP
    FARMAN, N
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1022 (01) : 126 - 128
  • [6] ABUNDANCE OF NA+-K+-ATPASE MESSENGER-RNA IS REGULATED BY GLUCOCORTICOID HORMONES IN INFANT RAT KIDNEYS
    CELSI, G
    NISHI, A
    AKUSJARVI, G
    APERIA, A
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1991, 260 (02): : F192 - F197
  • [7] DIFFERENTIAL REGULATION OF NA-K-ATPASE ISOFORM GENE-EXPRESSION BY T3 DURING RAT-BRAIN DEVELOPMENT
    CORTHESYTHEULAZ, I
    MERILLAT, AM
    HONEGGER, P
    ROSSIER, BC
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1991, 261 (01): : C124 - C131
  • [8] DETECTION OF MESSENGER-RNAS IN SEA-URCHIN EMBRYOS BY INSITU HYBRIDIZATION USING ASYMMETRIC RNA PROBES
    COX, KH
    DELEON, DV
    ANGERER, LM
    ANGERER, RC
    [J]. DEVELOPMENTAL BIOLOGY, 1984, 101 (02) : 485 - 502
  • [9] DOUCET A, 1986, J BIOL CHEM, V261, P993
  • [10] DIFFERENTIAL EXPRESSION OF NA+,K+-ATPASE ALPHA-SUBUNIT AND BETA-SUBUNIT MESSENGER-RNAS IN RAT-TISSUES AND CELL-LINES
    EMANUEL, JR
    GARETZ, S
    STONE, L
    LEVENSON, R
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (24) : 9030 - 9034