ADENOSINE-TRIPHOSPHATE AND ARACHIDONIC-ACID STIMULATE GLYCOGENOLYSIS IN PRIMARY CULTURES OF MOUSE CEREBRAL CORTICAL ASTROCYTES

Adenosine triphosphate (ATP) promotes glycogenolysis in primary cultures of mouse cerebral cortical astrocytes with an EC(50) of 1.5 mu M. A pharmacological analysis indicates an involvement of purinergic P-2Y receptors in this action of ATP. Application of either arachidonic acid (AA), or certain unsaturated fatty acids, also results in glycogen breakdown. The EC(50) of AA is approximately 50 mu M. Thus ATP and AA can be added to the list of neuroactive agents that control glycogen levels in astrocytes, which includes noradrenaline, vasoactive intestinal peptide (VIP), adenosine and histamine.