PERMEABILITY TO NONELECTROLYTES AS AN INDICATOR OF CHANGE IN MEMBRANE LIPID STRUCTURE IN HORDEUM-VULGARE CELLS INFECTED BY ERYSIPHE-GRAMINIS F SP HORDEI

被引:5
作者
LEESTADELMANN, OY
BUSHNELL, WR
CURRAN, CM
STADELMANN, EJ
机构
[1] UNIV MINNESOTA,USDA ARS,CEREAL RUST LAB,ST PAUL,MN 55108
[2] UNIV MINNESOTA,DEPT HORT SCI,PROTOPLASMATOL LAB,ST PAUL,MN 55108
关键词
D O I
10.1016/S0885-5765(05)80122-5
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The passive permeability to water and nonelectrolytes of epidermal cells from partially dissected coleoptiles of Hordeum vulagare was investigated in uninfected tissue and in tissue infected with a compatible race of the powdery mildew fungus, Erysiphe graminis f. sp. hordei. Permeability constants were determined plasmometrically for water and for the increasingly nonpolar electrolytes: urea, methyl urea, and ethyl urea. Measurements were taken 48 h after inoculation, usually in heavily infected tissues which exhibited concave plasmolysis. Permeability of cells to water or urea was unchanged by infection. Permeability to methyl urea was reduced significantly in the cultivar Montcalm, but not consistently in Atlas or AlgS. Permeability to ethyl urea in AlgS was reduced by half by infection. The reduced permeability occurred not only in haustoriumcontaining cells. but throughout heavily infected tissue. If tissues were left overnight on plasmolyzing solution of α-methyl glucose, uninfected tissues deplasmolyzed and regained turgor whereas infected tissue did not, suggesting that the α-methyl glucose entered healthy, but not infected cells. The results indicate that infection altered the plasmalemma of host cells, reducing passive permeability to α-methyl glucose and the more lipophilic of the nonelectrolytes tested. © 1991 Academic Press Limited.
引用
收藏
页码:163 / 178
页数:16
相关论文
共 37 条