STRUCTURAL AND THERMODYNAMIC STUDIES ON THE ADENINE GUANINE MISMATCH IN B-DNA

被引:102
作者
LEONARD, GA
BOOTH, ED
BROWN, T
机构
[1] Department of Chemistry, University of Edinburgh, Edinburgh EH9 3JJ, Kings Buildings, West Mains Road
关键词
D O I
10.1093/nar/18.19.5617
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The structure of the synthetic dodecamer d(CGCAAATTGGCG) has been shown by single crystal X-ray diffraction methods to be that of a B-DNA helix containing two A(anti).G(syn) base pairs. The refinement, based on data to a resolution of 2.25 A shows that the mismatch base pairs are held together by two hydrogen bonds. The syn-conformation of the guanine base of the mismatch is stabilised by hydrogen bonding to a network of solvent molecules in both the major and minor grooves. A pH-dependent ultraviolet melting study Indicates that the duplex is stabilised by protonation, suggesting that the bases of the A.G mispair are present in their most common tautomeric forms and that the N(1)-atom of adenine is protonated. The structure refinement shows that there is some disorder in the sugar-phosphate backbone. © 1990 Oxford University Press.
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页码:5617 / 5623
页数:7
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