HIGH CELL-DENSITY CULTIVATION OF ESCHERICHIA-COLI-W USING SUCROSE AS A CARBON SOURCE

被引：96

作者：

LEE, SY

CHANG, HN

机构：

[1] KOREA ADV INST SCI & TECHNOL, CTR BIOPROC ENGN RES, 373-1 KUSUNG DONG, YUSUNG GU, TAEJON 305701, SOUTH KOREA

[2] KOREA ADV INST SCI & TECHNOL, DEPT CHEM ENGN, TAEJON 305701, SOUTH KOREA

来源：

BIOTECHNOLOGY LETTERS | 1993年 / 15卷 / 09期

关键词：

D O I：

10.1007/BF00131766

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Escherichia coli W was cultivated in the pH-stat fed-batch mode in a chemically defined medium containing sucrose as a sole carbon source. A final cell concentration of 105 g/L could be obtained in 36 hrs by feeding 20 g sucrose+0.57 g MgSO47H2O for a definite on-time upon the pH rise. Using the same strategy, a recombinant strain harboring pSYL104 could also be grown to 125 g/L in 48 hrs.

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页码：971 / 974

页数：4

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