PCR ANALYSIS OF THE Y-CHROMOSOME LONG ARM IN AZOOSPERMIC PATIENTS - EVIDENCE FOR A 2ND LOCUS REQUIRED FOR SPERMATOGENESIS

被引：157

作者：

KOBAYASHI, K

MIZUNO, K

HIDA, A

KOMAKI, R

TOMITA, K

MATSUSHITA, I

NAMIKI, M

IWAMOTO, T

TAMURA, S

MINOWADA, S

NAKAHORI, Y

NAKAGOME, Y

机构：

[1] UNIV TOKYO,SCH INT HLTH,DEPT HUMAN GENET,BUNKYO KU,TOKYO 113,JAPAN

[2] OSAKA UNIV,SCH MED,DEPT UROL,OSAKA,JAPAN

[3] ST MARIANNA UNIV,SCH MED,DEPT UROL,KANAGAWA,JAPAN

[4] KEIO UNIV,SCH MED,DEPT OBSTET & GYNECOL,TOKYO,JAPAN

[5] UNIV TOKYO,FAC MED,DEPT UROL,TOKYO,JAPAN

来源：

HUMAN MOLECULAR GENETICS | 1994年 / 3卷 / 11期

基金：

日本科学技术振兴机构;

关键词：

D O I：

10.1093/hmg/3.11.1965

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We analyzed DNA from 63 Japanese men with either azoospermia or severe oligospermia whose Y chromosomes were cytogenetically normal. A total of 16 loci were examined: 15 loci on the long arm between DYS7E and DYZ1, and the YRRM1 locus, a candidate gene far the azoospermic factor, AZF. One patient with a pericentric inversion of the Y chromosome was also included. We detected micro-deletions in ten individuals. The YRRM1 gene was involved in only three of them, The remaining seven patients showed deletion between DYS7C and DYS239 in common, indicating the presence of at least one additional gene, deletion of which causes azoospermia.

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页码：1965 / 1967

页数：3

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