SENSE AND ANTISENSE MODIFICATION OF GLIAL ALPHA-B-CRYSTALLIN PRODUCTION RESULTS IN ALTERATIONS OF STRESS FIBER FORMATION AND THERMORESISTANCE

被引:89
作者
IWAKI, T
IWAKI, A
TATEISHI, J
GOLDMAN, JE
机构
[1] KYUSHU UNIV 18, GENET INFORMAT RES LAB, FUKUOKA 812, JAPAN
[2] COLUMBIA UNIV COLL PHYS & SURG, DEPT PATHOL, NEW YORK, NY 10032 USA
关键词
D O I
10.1083/jcb.125.6.1385
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The phenotypic effects of selectively altering the levels of alpha B-crystallin in cultured glial cells were analyzed using sense and antisense approaches. Rat C6 glioma cells and human U-373MG glioma cells were transfected with a rat alpha B-crystallin sense cDNA or an antisense cDNA regulated by a Rous sarcoma virus promoter to alter cellular levels of alpha B-crystallin. The antisense strategy resulted in decreased alpha B-crystallin levels, as revealed by Western blot and immunocytochemical analyses. The reduced alpha B-crystallin expression was accompanied by alterations in cellular phenotype: (a) a reduction of cell size and/or a slender cell morphology; (b) a disorganized microfilament network; and (c) a reduction of cell adhesiveness. Like HSP27, the presence of additional alpha B-crystallin protein confers a thermoresistant phenotype to stable transfectants. Thus, alpha B-crystallin in glioma cells plays a role in their thermal resistance and may contribute to the stability of cytoskeletal organization.
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页码:1385 / 1393
页数:9
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