OXYGEN-REGULATED IN-VITRO TRANSCRIPTION OF RHIZOBIUM-MELILOTI NIFA AND FIXK GENES

被引：72

作者：

REYRAT, JM

DAVID, M

BLONSKI, C

BOISTARD, P

BATUT, J

机构：

[1] INRA,CNRS,BIOL MOLEC RELAT PLANTES MICROORGANISMES LAB,BP 27,F-31326 CASTANET TOLOSAN,FRANCE

[2] UNIV TOULOUSE 3,CHIM ORGAN BIOL GRP,CNRS,URA 470,F-31062 TOULOUSE,FRANCE

来源：

JOURNAL OF BACTERIOLOGY | 1993年 / 175卷 / 21期

关键词：

D O I：

10.1128/jb.175.21.6867-6872.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Oxygen concentration regulates the expression of nitrogen fixation genes in the symbiotic bacterium Rhizobium meliloti. We demonstrate that two proteins, FixL and FixJ, that belong to the two-component family of regulatory proteins are necessary and sufficient for oxygen-regulated in vitro transcription of the two key regulatory genes, nifA and fixK. We show directly that FixJ is a transcriptional activator, working in conjunction with the RNA polymerase sigma 70 holoenzyme. Addition of FixL122, a soluble form of the sensor FixL protein, to the transcription assay enhanced FixJ transcriptional activity in response to low oxygen concentration. This enhancement of FixJ activity was correlated with FixJ phosphorylation.

引用

收藏

页码：6867 / 6872

页数：6

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