POTENTIATION OF CYTOTOXICITY OF MITOXANTRONE TOWARD CHO-K1 CELLS-INVITRO BY DIPYRIDAMOLE

被引:5
作者
DESAI, PB
SRIDHAR, R
机构
[1] HOWARD UNIV, DEPT RADIAT THERAPY, WASHINGTON, DC 20060 USA
[2] UNIV S CAROLINA, COLL PHARM, DEPT BASIC PHARMACEUT SCI, COLUMBIA, SC 29208 USA
[3] HOWARD UNIV, CTR CANC, WASHINGTON, DC 20060 USA
关键词
MITOXANTRONE; CYTOTOXICITY; CHEMOSENSITIZATION; CHO-K1; CELLS; DIPYRIDAMOLE;
D O I
10.1023/A:1018920903436
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Dipyridamole (DP), a clinically used vasodilator and an antiplatelet compound, augmented the activity of the anticancer drug mitoxantrone (MXN) toward chinese hamster ovary (CHO-K1) cells in culture. Clonogenic assays indicated that DP (1.0, 2.5, and 5.0-mu-M) decreased the survival of cells treated with MXN (5 to 25 nM in a dose-dependent manner. Further, DP (1 and 5-mu-M) decreased the MXN concentration required for 50% inhibition of cell growth from 3.2 to 1.8 and from 3.0 to 0.5 nM, respectively, over a period of 3 days. DP (10-mu-M) increased the accumulation of MXN by 1.8-fold in exponentially growing cells exposed to MXN. The enhanced levels of MXN in CHO-K1 cells in the presence of the chemosensitizer may account for the potentiation of MXN-cytotoxicity by DP.
引用
收藏
页码:178 / 181
页数:4
相关论文
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