CLONING OF A HUMAN CHOLINE KINASE CDNA BY COMPLEMENTATION OF THE YEAST CKI MUTATION

被引：40

作者：

HOSAKA, K ^{[1
]}

TANAKA, S ^{[1
]}

NIKAWA, J ^{[1
]}

YAMASHITA, S ^{[1
]}

机构：

[1] GUNMA UNIV,SCH MED,DEPT BIOCHEM,MAEBASHI,GUNMA 371,JAPAN

来源：

FEBS LETTERS | 1992年 / 304卷 / 2-3期

关键词：

HUMAN CHOLINE KINASE CDNA; FUNCTIONAL CLONING; YEAST MUTANT;

D O I：

10.1016/0014-5793(92)80625-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A human choline kinase cDNA was cloned by complementation of the yeast choline kinase mutation, cki, from a human glioblastoma cDNA expression library. The deduced sequence of the human enzyme comprised 456 amino acids with a calculated relative molecular mass of 52,065. The human enzyme resembled the rat liver enzyme over the entire sequence. It also resembled the yeast enzyme in the carboxy-terminal region, but not much in the amino-terminal region.

引用

页码：229 / 232

页数：4

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