CLONING OF A HUMAN CHOLINE KINASE CDNA BY COMPLEMENTATION OF THE YEAST CKI MUTATION

被引：40

作者：

HOSAKA, K ^{[1
]}

TANAKA, S ^{[1
]}

NIKAWA, J ^{[1
]}

YAMASHITA, S ^{[1
]}

机构：

[1] GUNMA UNIV,SCH MED,DEPT BIOCHEM,MAEBASHI,GUNMA 371,JAPAN

来源：

FEBS LETTERS | 1992年 / 304卷 / 2-3期

关键词：

HUMAN CHOLINE KINASE CDNA; FUNCTIONAL CLONING; YEAST MUTANT;

D O I：

10.1016/0014-5793(92)80625-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A human choline kinase cDNA was cloned by complementation of the yeast choline kinase mutation, cki, from a human glioblastoma cDNA expression library. The deduced sequence of the human enzyme comprised 456 amino acids with a calculated relative molecular mass of 52,065. The human enzyme resembled the rat liver enzyme over the entire sequence. It also resembled the yeast enzyme in the carboxy-terminal region, but not much in the amino-terminal region.

引用

页码：229 / 232

页数：4

共 24 条

[11] EXPRESSION OF THE HIS3 GENE OF SACCHAROMYCES-CEREVISIAE IN POLYNUCLEOTIDE PHOSPHORYLASE-DEFICIENT STRAINS OF ESCHERICHIA-COLI K-12 [J].

KASUNIC, DA ;

KUSHNER, SR .

GENE, 1980, 12 (1-2) :1-10

[12]

KELLEY MJ, 1988, J BIOL CHEM, V263, P18078

[13] REGULATION OF PHOSPHATIDYLCHOLINE BIOSYNTHESIS [J].

KENT, C .

PROGRESS IN LIPID RESEARCH, 1990, 29 (02) :87-105

[14] COMPLEMENTATION USED TO CLONE A HUMAN HOMOLOG OF THE FISSION YEAST-CELL CYCLE CONTROL GENE CDC2 [J].

LEE, MG ;

NURSE, P .

NATURE, 1987, 327 (6117) :31-35

[15] A SYSTEM FOR SHOTGUN DNA SEQUENCING [J].

MESSING, J ;

CREA, R ;

SEEBURG, PH .

NUCLEIC ACIDS RESEARCH, 1981, 9 (02) :309-321

[16] CHARACTERIZATION OF PHOSPHATIDYLSERINE SYNTHASE FROM SACCHAROMYCES-CEREVISIAE AND A MUTANT DEFECTIVE IN THE ENZYME [J].

NIKAWA, J ;

YAMASHITA, S .

BIOCHIMICA ET BIOPHYSICA ACTA, 1981, 665 (03) :420-426

[17] YEAST MUTANT DEFECTIVE IN SYNTHESIS OF PHOSPHATIDYLINOSITOL - ISOLATION AND CHARACTERIZATION OF A CDP-DIACYLGLYCEROL-INOSITOL 3-PHOSPHATIDYLTRANSFERASE KM MUTANT [J].

NIKAWA, JI ;

YAMASHITA, S .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1982, 125 (02) :445-451

[18]

PORTER TJ, 1990, J BIOL CHEM, V265, P414

[19] DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS [J].

SANGER, F ;

NICKLEN, S ;

COULSON, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5463-5467

[20] CLONING OF 3 HUMAN MULTIFUNCTIONAL DENOVO PURINE BIOSYNTHETIC GENES BY FUNCTIONAL COMPLEMENTATION OF YEAST MUTATIONS [J].

SCHILD, D ;

BRAKE, AJ ;

KIEFER, MC ;

YOUNG, D ;

BARR, PJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (08) :2916-2920

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