MAGNETIC-RESONANCE-IMAGING AND SPECTROSCOPY OF MCF-7 HUMAN BREAST-CANCER - PATHOPHYSIOLOGY AND MONITORING OF TREATMENT

Magnetic resonance imaging at high spatial resolution, dynamic-contrast enhanced imaging, and localized P-31 magnetic resonance spectroscopy were applied to monitor in vivo growth and tamoxifen treatment of MCF7 human breast tumors implanted in athymic mice. Correlation of the imaging data with hostological findings demonstrated significant differences between viable carcinoma, fibrous, and necrotic regions on T-2 weighted images. At an early stage after tamoxifen treatment rapid necrosis preceded tumor regression suggesting tamoxifen inhibition of angiogenesis. The necrosis was followed by growth of reperative fibrous tissue at the boundary of the viable cancer cells and the necrotic regions. The dynamic of contrast enhancement after tamoxifen treatment revealed high permeability of microvessels at this boundary cord. In parallel to the induction of the repair process the tumor energy profile changed, showing a significant increase in the ratio of ATP to inorganic phosphate. Initial attempts to use MRI for evaluating the response of breast cancer patients to treatment are also described.