STUDIES ON THE EXCHANGE OF VALINE-OXYGEN DURING THE BIOSYNTHESIS OF DELTA-(L-ALPHA-AMINOADIPOYL)-L-CYSTEINYL-D-VALINE

被引：6

作者：

BALDWIN, JE ^{[1
]}

ADLINGTON, RM ^{[1
]}

BIRD, JW ^{[1
]}

FIELD, RA ^{[1
]}

OCALLAGHAN, NM ^{[1
]}

SCHOFIELD, CJ ^{[1
]}

机构：

[1] UNIV OXFORD,OXFORD CTR MOLEC SCI,OXFORD OX1 3QY,ENGLAND

来源：

TETRAHEDRON | 1992年 / 48卷 / 06期

关键词：

CEPHALOSPORIUM ACREMONIUM; BIOSYNTHESIS; DELTA-(L-ALPHA-AMINOADIPOYL)-L-CYSTEINYL-D-VALINE (ACV); 18O2]-VALINE INCORPORATION; PENICILLIN;

D O I：

10.1016/S0040-4020(01)88205-3

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Incorporation of [4-H-2(6),O-18(2)]-valine into delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine (ACV), by intact cells of Cephalosporium acremonium, demonstrated the intracellular exchange of one and both valine oxygen atoms. Incubation of [O-18(2)]-valine with the purified ACV synthetase from C. acremonium gave exclusive incorporation of a single O-18 label into ACV, consistent with the effectively non-reversible formation of a covalent valinoyl-ACV synthetase intermediate under in vitro conditions.

引用

页码：1099 / 1108

页数：10

共 18 条

[1] CONVERSION OF O-17/O-18 LABELED DELTA-(L-ALPHA-AMINOADIPYL)-L-CYSTEINYL-D-VALINE TO O-17/O-18 LABELED ISOPENICILLIN-N IN A CELL-FREE-EXTRACT OF CEPHALOSPORIUM ACREMONIUM - A STUDY BY O-17-NMR SPECTROSCOPY AND MASS-SPECTROMETRY [J].

ADLINGTON, RM ;

APLIN, RT ;

BALDWIN, JE ;

CHAKRAVARTI, B ;

FIELD, LD ;

JOHN, EMM ;

ABRAHAM, EP ;

WHITE, RL .

TETRAHEDRON, 1983, 39 (07) :1061-1068

[2]

[Anonymous], BIOCH PEPTIDE ANTIBI

[3] ISOLATION AND PARTIAL CHARACTERIZATION OF ACV SYNTHETASE FROM CEPHALOSPORIUM-ACREMONIUM AND STREPTOMYCES-CLAVULIGERUS - EVIDENCE FOR THE PRESENCE OF PHOSPHOPANTOTHENATE IN ACV SYNTHETASE [J].

BALDWIN, JE ;

BIRD, JW ;

FIELD, RA ;

OCALLAGHAN, NM ;

SCHOFIELD, CJ ;

WILLIS, AC .

JOURNAL OF ANTIBIOTICS, 1991, 44 (02) :241-248

[4] EXCHANGE OF VALINE-OXYGEN DURING THE BIOSYNTHESIS OF DELTA-(L-ALPHA-AMINOADIPOYL)-L-CYSTEINYL-D-VALINE [J].

BALDWIN, JE ;

FIELD, RA ;

SCHOFIELD, CJ .

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1991, (21) :1531-1532

[5] THE BIOSYNTHESIS OF PENICILLINS AND CEPHALOSPORINS [J].

BALDWIN, JE ;

ABRAHAM, E .

NATURAL PRODUCT REPORTS, 1988, 5 (02) :129-145

[6] THE FATE OF VALINE-OXYGEN DURING PENICILLIN BIOSYNTHESIS [J].

BALDWIN, JE ;

ADLINGTON, RM ;

BIRD, JW ;

SCHOFIELD, CJ .

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1989, (21) :1615-1616

[7] PENICILLIN BIOSYNTHESIS - RETENTION OF CONFIGURATION AT C-3 OF VALINE DURING ITS INCORPORATION INTO THE ARNSTEIN TRIPEPTIDE [J].

BALDWIN, JE ;

WAN, TS .

TETRAHEDRON, 1981, 37 (08) :1589-1595

[8]

BALDWIN JE, UNPUB TETRAHEDRON

[9] DELTA-(L-ALPHA-AMINOADIPYL)-L-CYSTEINYL-D-VALINE SYNTHETASE (ACV SYNTHETASE) - A MULTIFUNCTIONAL ENZYME WITH BROAD SUBSTRATE-SPECIFICITY FOR THE SYNTHESIS OF PENICILLIN AND CEPHALOSPORIN PRECURSORS [J].

BANKO, G ;

DEMAIN, AL ;

WOLFE, S .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1987, 109 (09) :2858-2860

[10] PARTIAL ELIMINATION OF VALINE-OXYGEN DURING PENICILLIN BIOSYNTHESIS [J].

DELDERFIELD, JS ;

MTETWA, E ;

THOMAS, R ;

TYOBEKA, TE .

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1981, (13) :650-652

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