EXPRESSION OF 3 HUMAN BETA-ADRENERGIC-RECEPTOR SUBTYPES IN TRANSFECTED CHINESE-HAMSTER OVARY CELLS

被引：128

作者：

TATE, KM ^{[1
]}

BRIENDSUTREN, MM ^{[1
]}

EMORINE, LJ ^{[1
]}

DELAVIERKLUTCHKO, C ^{[1
]}

MARULLO, S ^{[1
]}

STROSBERG, AD ^{[1
]}

机构：

[1] UNIV PARIS 07,INST COCHIN GENET MOLEC,IMMUNOPHARMACOL MOLEC LAB,CNRS,22 RUE MECHAIN,F-75014 PARIS,FRANCE

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1991年 / 196卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1991.tb15824.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The genes coding for three pharmacologically distinct subtypes of human beta-adrenergic receptors (beta-1AR, beta-2AR and beta-3AR) were transfected for expression in Chinese hamster ovary (CHO) cells. Stable cell lines expressing each receptor were analyzed by ligand binding, adenylate cyclase activation and photoaffinity labeling, and compared to beta-AR subtypes observed in previously described tissues, primary cultures and transfected cell lines. Each of the three receptor subtypes displayed saturable [I-125]iodocyanopindolol-binding activity. They showed the characteristic rank order of potencies for five agonists, determined by measuring the accumulation of intracellular cAMP. These recombinant cell lines express a homogeneous population of receptors and display the known pharmacological properties of beta-1AR and beta-2AR, in human tissues. It is therefore likely that the pattern of ligand binding and adenylate cyclase activation, mediated by the new beta-3AR in CHO cells, also reflects the yet-undetermined pharmacological profile in humans.

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页码：357 / 361

页数：5

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