RAPID DETECTION OF MUMPS-VIRUS BY THE POLYMERASE CHAIN-REACTION

被引：17

作者：

BORISKIN, YS ^{[1
]}

BOOTH, JC ^{[1
]}

YAMADA, A ^{[1
]}

机构：

[1] NATL INST HLTH,DEPT MEASLES VIRUS,TOKYO 141,JAPAN

来源：

JOURNAL OF VIROLOGICAL METHODS | 1993年 / 42卷 / 01期

基金：

英国惠康基金;

关键词：

PARAMYXOVIRUS; RNA-PCR; RAPID DIAGNOSIS;

D O I：

10.1016/0166-0934(93)90173-O

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure for detecting mumps virus in under 48 h was developed using the PCR. The sensitivity of the PCR amplification reaction and of the detection of the PCR product was significantly improved by: (i) enriching for viral template RNAs by overnight culture of the virus in Vero cells and (ii) substitution of polyacrylamide gel analysis for agarose gel electrophoresis. The technique was capable of detecting 1-20 infectious units of virus or an equivalent of 1-10 pg of mumps virus-specific plasmid DNA.

引用

页码：23 / 32

页数：10