CHARACTERIZATION AND PARTIAL-PURIFICATION FROM PHEOCHROMOCYTOMA CELLS OF AN ENDOGENOUS EQUIVALENT OF SCYLLATOXIN, A SCORPION TOXIN WHICH BLOCKS SMALL CONDUCTANCE CA2+-ACTIVATED K+ CHANNELS

被引：9

作者：

AUGUSTE, P ^{[1
]}

HUGUES, M ^{[1
]}

BORSOTTO, M ^{[1
]}

THIBAULT, J ^{[1
]}

ROMEY, G ^{[1
]}

COPPOLA, T ^{[1
]}

LAZDUNSKI, M ^{[1
]}

机构：

[1] INST PHARMACOL MOLEC & CELLULAIRE,660 ROUTE LUCIOLES,F-06560 VALBONNE,FRANCE

来源：

BRAIN RESEARCH | 1992年 / 599卷 / 02期

关键词：

IONIC CHANNEL; ENDOGENOUS LIGAND; PHEOCHROMOCYTOMA; PURIFICATION; PEPTIDE; TOXIN; VENOM;

D O I：

10.1016/0006-8993(92)90396-Q

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

This work describes the partial purification of a heat-stable peptide which has the same properties as the scorpion toxin, scyllatoxin, a specific blocker of one class of Ca2+-activated K+ channels: (i) it competes with [I-125]apamin for binding to the same site, (ii) like apamin and scyllatoxin, it blocks the after-potential hyperpolarization in skeletal muscle cells in culture, (iii) like apamin and scyllatoxin, it contracts guinea-pig taenia coli relaxed by epinephrine, (iv) it cross-reacts with antibodies raised against scyllatoxin but not with antibodies raised against apamin.

引用

页码：230 / 236

页数：7

共 34 条

[11] PURIFICATION, SEQUENCE, AND MODEL STRUCTURE OF CHARYBDOTOXIN, A POTENT SELECTIVE INHIBITOR OF CALCIUM-ACTIVATED POTASSIUM CHANNELS [J].

GIMENEZGALLEGO, G ;

NAVIA, MA ;

REUBEN, JP ;

KATZ, GM ;

KACZOROWSKI, GJ ;

GARCIA, ML .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (10) :3329-3333

[12] DYNORPHIN-(1-13), AN EXTRAORDINARILY POTENT OPIOID PEPTIDE [J].

GOLDSTEIN, A ;

TACHIBANA, S ;

LOWNEY, LI ;

HUNKAPILLER, M ;

HOOD, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (12) :6666-6670

[13] IDENTIFICATION OF 2 RELATED PENTAPEPTIDES FROM BRAIN WITH POTENT OPIATE AGONIST ACTIVITY [J].

HUGHES, J ;

SMITH, TW ;

KOSTERLITZ, HW ;

FOTHERGILL, LA ;

MORGAN, BA ;

MORRIS, HR .

NATURE, 1975, 258 (5536) :577-579

[14] APAMIN AS A SELECTIVE BLOCKER OF THE CALCIUM-DEPENDENT POTASSIUM CHANNEL IN NEURO-BLASTOMA CELLS - VOLTAGE-CLAMP AND BIOCHEMICAL-CHARACTERIZATION OF THE TOXIN RECEPTOR [J].

HUGUES, M ;

ROMEY, G ;

DUVAL, D ;

VINCENT, JP ;

LAZDUNSKI, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (04) :1308-1312

[15]

HUGUES M, 1982, J BIOL CHEM, V257, P2762

[16]

HUGUES M, 1982, LIFE SCI, V31, P437, DOI 10.1016/0024-3205(82)90328-9

[17] CLONING AND SEQUENCE-ANALYSIS OF CDNA-ENCODING THE PRECURSOR OF A HUMAN ENDOTHELIUM-DERIVED VASOCONSTRICTOR PEPTIDE, ENDOTHELIN - IDENTITY OF HUMAN AND PORCINE ENDOTHELIN [J].

ITOH, Y ;

YANAGISAWA, M ;

OHKUBO, S ;

KIMURA, C ;

KOSAKA, T ;

INOUE, A ;

ISHIDA, N ;

MITSUI, Y ;

ONDA, H ;

FUJINO, M ;

MASAKI, T .

FEBS LETTERS, 1988, 231 (02) :440-444

[18] APAMIN, A NEUROTOXIN SPECIFIC FOR ONE CLASS OF CA-2+-DEPENDENT K+ CHANNELS [J].

LAZDUNSKI, M .

CELL CALCIUM, 1983, 4 (5-6) :421-428

[19] NA+ CHANNELS WITH BINDING-SITES OF HIGH AND LOW AFFINITY FOR TETRODOTOXIN IN DIFFERENT EXCITABLE AND NON-EXCITABLE CELLS [J].

LOMBET, A ;

FRELIN, C ;

RENAUD, JF ;

LAZDUNSKI, M .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1982, 124 (01) :199-203

[20] IDENTIFICATION IN MAMMALIAN BRAIN OF AN ENDOGENOUS SUBSTANCE WITH NA+ CHANNEL BLOCKING ACTIVITIES SIMILAR TO THOSE OF TETRODOTOXIN [J].

LOMBET, A ;

FOSSET, M ;

ROMEY, G ;

JACOMET, Y ;

LAZDUNSKI, M .

BRAIN RESEARCH, 1987, 417 (02) :327-334

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