THE LYSOSOMAL GP63-RELATED PROTEIN IN LEISHMANIA-MEXICANA AMASTIGOTES IS A SOLUBLE METALLOPROTEINASE WITH AN ACIDIC PH OPTIMUM

被引：44

作者：

ILG, T

HARBECKE, D

OVERATH, P

机构：

[1] Max-Planck-Institut für Biologie, D-72076 Tübingen

来源：

FEBS LETTERS | 1993年 / 327卷 / 01期

关键词：

METALLOPROTEINASE; CYSTEINE PROTEINASE; LYSOSOME; LEISHMANIA-MEXICANA;

D O I：

10.1016/0014-5793(93)81049-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Leishmania mexicana amastigotes express a lysosomal protein, which is antigenically related to the promastigote surface metalloproteinase (gp63). It is shown that the purified gp63-related protein from amastigote is also an active metalloproteinase. The pH-optimum of the enzyme is acidic, similar to lysosomal cysteine proteinases, but distinct from the neutral to basic pH-optimum of the promastigote surface proteinase. This study appears to be the first report on a metalloproteinase with a lysosomal localization.

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页码：103 / 107

页数：5

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