TRICHORZINS HA AND MA, ANTIBIOTIC PEPTIDES FROM TRICHODERMA-HARZIANUM .1. FERMENTATION, ISOLATION AND BIOLOGICAL PROPERTIES

被引：45

作者：

GOULARD, C ^{[1
]}

HLIMI, S ^{[1
]}

REBUFFAT, S ^{[1
]}

BODO, B ^{[1
]}

机构：

[1] MUSEUM NATL HIST NAT,CHIM SUBST NAT LAB,CNRS,GDR 1153,CNRS,URA 401,F-75231 PARIS 05,FRANCE

来源：

JOURNAL OF ANTIBIOTICS | 1995年 / 48卷 / 11期

关键词：

D O I：

10.7164/antibiotics.48.1248

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Trichorzins HA and MA, original 18-residue peptides, were isolated from two strains of the widespread soil fungus Trichoderma harzianum which have been shown to exhibit antibiotic activity against phytopathogenic fungi. These linear peptides belonging to the peptaibol class are biosynthesized as a complex of closely related analogues. Nine major pure peptides, six trichorzins HA and three trichorzins MA, were isolated by reversed-phase HPLC. The isolated peptides exhibited antibacterial activity against S. aureus and increased the membrane permeability of egg phosphatidylcholine/cholesterol (7/3) liposomes, as measured by monitoring leakage kinetics of a fluorescent probe. Structure-activity relationships were deduced from the antibiotic and membrane-modifying properties.

引用

页码：1248 / 1253

页数：6