DIRECT IDENTIFICATION AND CHARACTERIZATION OF LLAMA (LAMA-GLAMA L) WHEY PROTEINS BY MICROSEQUENCING AFTER WESTERN BLOTTING

被引：17

作者：

CANTISANI, A ^{[1
]}

NAPOLITANO, L ^{[1
]}

GIUFFRIDA, MG ^{[1
]}

CONTI, A ^{[1
]}

机构：

[1] INST CHEM,CNR,CTR STUDI ALIMENTAZ ANIM,VIA P GIURIA 7,I-10126 TURIN,ITALY

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1990年 / 21卷 / 03期

关键词：

Llama; Microsequencing; Western blotting; Whey protein;

D O I：

10.1016/0165-022X(90)90016-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Amino acid sequence determination is the most reliable and powerful tool to identify a protein or to classify a new one by comparison of its primary structure with already known sequences. A rapid and simple purification procedure is an essential pre-requisite for routine sequence determination. Structural characterization of llama whey proteins was undertaken for evolutionary as well as economic purposes. N-terminal sequence analyses directly on an immobilon polyvinylidene difluoride (PVDF) membrane, following Western blotting of both native and SDS-denatured llama whey proteins after polyacrylamide gel electrophoresis, revealed three different forms of glycosylated α-lactalbumin, and a protein with a high degree of homology with a camel whey protein of unknown function. Furthermore, by immunoblotting techniques, the electrophoretic band corresponding to serum albumin was identified. © 1990.

引用

页码：227 / 236

页数：10